Cover Page
The handle http://hdl.handle.net/1887/45030 holds various files of this Leiden University dissertation
Author: Schendel, Robin van
Title: Alternative end-joining of DNA breaks
Issue Date: 2016-12-15
Alternative end-joining of DNA breaks
Robin van Schendel
Cover design & Layout: Robin van Schendel
Printing: Off Page, www.offpage.nl
ISBN: 978-94-6182-741-8
© Copyright 2016 by Robin van Schendel. All rights reserved
No part of this book may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, without prior permission of the author, or when appropriate, of the publisher of the presented articles.
Alternative end-joining of DNA breaks
Proefschrift
ter verkrijging van
de graad van Doctor aan de Universiteit Leiden, op gezag van Rector Magnificus prof.mr. C.J.J.M. Stolker,
volgens besluit van het College voor Promoties te verdedigen op donderdag 15 december 2016
klokke 13.45
door
Robin van Schendel geboren te Rijswijk
in 1983
Promotiecommissie
Promotoren: Prof. dr. M. Tijsterman
Prof. dr. J. Brouwer
Leden Promotiecommissie: Prof. J. den Dunnen
Prof. dr. H. te Riele (NKI, Amsterdam)
Dr. P. Knipscheer (Hubrecht Institute, Utrecht)
TABLE OF CONTENTS
Chapter 1 General introduction 7
Chapter 2 Microhomology-mediated intron loss (MMIL) during metazoan evolution
25
Chapter 3 Polymerase theta-mediated end joining of replication-associated
DNA breaks in C. elegans 41
Chapter 4 Polymerase Θ is a key driver of genome evolution and of
CRISPR/Cas9-mediated mutagenesis 67
Chapter 5 Genomic scars generated by polymerase theta reveal the versatile mechanism of alternative end-joining
93
Chapter 6 General discussion and future perspectives 121
Appendix Summary Samenvatting Curriculum Vitae Publications Acknowledgements
131 133 139 141 143
