In silico and wet lab approaches to study transcriptional regulation
Hestand, M.S.
Citation
Hestand, M. S. (2010, June 29). In silico and wet lab approaches to study transcriptional regulation. Retrieved from https://hdl.handle.net/1887/15753
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License: Licence agreement concerning inclusion of doctoral thesis in the Institutional Repository of the University of Leiden
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In Silico and Wet Lab Approaches to Study Transcriptional Regulation
Proefschrift ter verkrijging van
de graad van Doctor aan de Universiteit Leiden,
op gezag van de Rector Magnificus Prof. mr. P.F. van der Heijden, volgens besluit van het College voor Promoties
te verdedigen op dinsdag 29 juni 2010 klokke 11:15 uur
door
Matthew Scott Hestand
geboren te Lexington, Kentucky, USA in 1979
Promotion committee
Promotores: Prof.dr. G.J.B. van Ommen Prof.dr. J.T. den Dunnen Co-promoter: Dr. P.A.C. ’t Hoen
Overige leden: Dr. B. van Steensel (NKI) Prof.dr. P. de Knijff Dr. A.A.F. de Vries
The research described in this thesis was performed in the Department of Human Genetics, Leiden University Medical Center, The Netherlands. Chapter 3 research was in collaboration with the EMBL-European Bioinformatics Institute, Wellcome Trust Genome Campus, Cambridge CB10 1SD, UK.
This work was supported by the Centre for Biomedical Genetics, the Netherlands, and the Centre for Medical Systems Biology, co-funded by the Netherlands Genome Initiative, and received additional funding by a Marie Curie Fellowship.
Printing of this thesis was supported by the Centre for Biomedical Genetics and the Netherlands Bioinformatics Centre (NBIC).
Printed by: Gildeprint Drukkerijen ISBN: 978-94-6108-057-8
(C) 2010 Matthew S. Hestand, Leiden, The Netherlands except (parts of )
Chapter 2: (C) 2008 Hestand et al; licensee BioMed Central Ltd.
Chapter 5: (C) Ramos et al. 2010. Published by Oxford University Press.
All rights reserved. No part of this thesis may be reproduced or transmitted in any form or by any means, electronic or mechanical, without prior permission of the author.
Contents
1 Introduction 7
2 CORE TF 19
3 Enrichment with Sunflower 45
4 GAPSS 63
5 CBP/p300 ChIP-seq 71
6 CAGE/SAGE: muscle gene structure 101
7 Discussion 125
8 Summary 133
9 Samenvatting 135
Abbreviations 139
Bibliography 141
Publications 155
CV 157
5
Contents
6
