University of Groningen Enzyme engineering for sustainable production of caprolactam Marjanovic, Antonija

University of Groningen

Enzyme engineering for sustainable production of caprolactam Marjanovic, Antonija

DOI:

10.33612/diss.168442979

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Publication date: 2021

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Marjanovic, A. (2021). Enzyme engineering for sustainable production of caprolactam. University of Groningen. https://doi.org/10.33612/diss.168442979

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1. - Since there is sufficient knowledge on enzyme reactions, the field of biocatalysis should proceed away from the question of ‘why enzymes work the way they do’ towards ‘how can we use their potential’ in order to tackle relevant societal challenges.

2. -Optimization of artificial metabolic pathways suffers from the Butterfly Effect: small irregularities can yield divergent results (Chapter 2).

3. - Analysis of the substrate profile of small rationally designed mutant libraries by molecular simulations can improve

computational design protocols of PLP-dependent enzymes. (Chapter 3)

4. - Altering substrate specificity of DAPDH by focusing on first- and second-shell active site residues will not yield efficient variants for implementation in an artificial metabolic pathway. (Chapter 4) 5. - The scientific value of an X-ray structure should not be

determined by the resolution of the crystal only. Even at a low resolution and without substrates bound to it, a new X-ray structure can provoke interesting new questions. (Chapter 5)

6. -Protein engineering can only be called successful, when at the end a bottle of powder can be put on the table.

7. - The biggest success and pitfall of the human race is rooted in the wish to separate.

8. -Disruptive action starts not when we think we will run off the cliff eventually, but when we see the edge of the cliff and the depths behind it and we accept the truth that the time to act is now.