University of Groningen
Human cell-based in vitro systems for vaccine evaluation
Tapia Calle, María Gabriela
DOI:
10.33612/diss.100812074
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Publication date:
2019
Link to publication in University of Groningen/UMCG research database
Citation for published version (APA):
Tapia Calle, M. G. (2019). Human cell-based in vitro systems for vaccine evaluation. University of
Groningen. https://doi.org/10.33612/diss.100812074
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1. Intrinsic immunological differences between animals and humans result in poor translation of vaccine responses from animal models to the human setting. Hence, alternative approaches that can help increase the odds of vaccines in succeeding in clinical trials are urgently needed.
2. Genomics and systems biology have fuelled advances in our understanding of human immunology. Together with adjuvant development and structure-based design of immunogens, these next-generation technologies are transforming the field of vaccinology and shaping the future of medicine.
3. Primary cells, irrespective whether they are derived from fresh or frozen PBMCs are a robust and reproducible platform to assess the immunogenicity of vaccine candidates and vaccine-induced immune mechanisms. (This thesis)
4. Human primary Mo-DCs and T cells are capable of responding to vaccines in measurable and consistent ways even if the vaccines are inactivated and non-adjuvanted. (This thesis)
5. An in vitro platform using long-term cultures of PBMCs is suitable to identify distinctive responses of human T cells, including T follicular helper cells to vaccines and vaccine candidates. (This thesis)
6. An in vitro vaccine evaluation system, which focusses on innate (DCs) and adaptive (T cells) responses could potentially serve four purposes: assess the quality of vaccines batches, select promising vaccine candidates, reveal vaccine mechanisms and help identifying ways to improve protective responses. (This thesis)
7. “Productivity is for robots. Humans excel at wasting time, experimenting, playing, creating, and exploring.” – Kevin Kelly
8. “In preparing for battle, I have found that planning is essential, but plans are useless.” – Dwight D. Eisenhower
9. “Cultural and religious stereotypes are useless for understanding the world.” – Hans Rosling, Factfulness
