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University of Groningen

The versatile activity of glycogen branching enzymes

Gänssle, Lucie

DOI:

10.33612/diss.134377482

IMPORTANT NOTE: You are advised to consult the publisher's version (publisher's PDF) if you wish to cite from

it. Please check the document version below.

Document Version

Publisher's PDF, also known as Version of record

Publication date:

2020

Link to publication in University of Groningen/UMCG research database

Citation for published version (APA):

Gänssle, L. (2020). The versatile activity of glycogen branching enzymes. University of Groningen.

https://doi.org/10.33612/diss.134377482

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Downloaded from the University of Groningen/UMCG research database (Pure): http://www.rug.nl/research/portal. For technical reasons the number of authors shown on this cover page is limited to 10 maximum.

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The versatile activity of

glycogen branching enzymes

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Cover design: Gisela Scheubmayr and Lucie Gaenssle

Printed by: Ipskamp Printing

The work described in this thesis was performed in the group of Bioproduct

Engineering of the Engineering and Technology Institue Groningen (ENTEG) in

the University of Groningen. This research was supported by the Netherlands

Organization for Scientific Research (NWO Groen program) and financially

supported by AVEBE.

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The versatile activity of

glycogen branching

enzymes

PhD thesis

to obtain the degree of PhD at the

University of Groningen

on the authority of the

Rector Magnificus Prof. C. Wijmenga

and in accordance with

the decision by the College of Deans.

This thesis will be defended in public on

Friday 16 October 2020 at 12.45 p.m

by

Aline Lucie Odette Gaenssle

born on 20 April 1990

in Vienna, Austria

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Supervisor

Prof. M.J.E.C. van der Maarel

Co-supervisors

Dr. E. Jurak

Dr. R.J. Leemhuis

Assessment committee

Prof. C. D'Hulst

Prof. M.W. Fraaije

Prof. H.A. Schols

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Table of contents

Chapter 1

General introduction...7

Chapter 2

Reliability factor for identification of low activity of amylolytic enzymes in the starch-iodine assay...45

Chapter 3

The degree of branching of amylopectin is positively correlated to gelatinized starch digestibility...65

Chapter 4

The structure of the final product of glycogen branching enzymes will be achieved irrespective of the substrate structure...85

Chapter 5

GH13 Glycogen branching enzymes can adapt the substrate chain length towards their preferences via α-1,4-transglycosylation...107

Chapter 6

Summary, discussion and conclusion...127

Samenvatting...143

Acknowledgments...147

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