University of Groningen
The versatile activity of glycogen branching enzymes
Gänssle, Lucie
DOI:
10.33612/diss.134377482
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Publication date:
2020
Link to publication in University of Groningen/UMCG research database
Citation for published version (APA):
Gänssle, L. (2020). The versatile activity of glycogen branching enzymes. University of Groningen.
https://doi.org/10.33612/diss.134377482
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The versatile activity of
glycogen branching enzymes
Cover design: Gisela Scheubmayr and Lucie Gaenssle
Printed by: Ipskamp Printing
The work described in this thesis was performed in the group of Bioproduct
Engineering of the Engineering and Technology Institue Groningen (ENTEG) in
the University of Groningen. This research was supported by the Netherlands
Organization for Scientific Research (NWO Groen program) and financially
supported by AVEBE.
The versatile activity of
glycogen branching
enzymes
PhD thesis
to obtain the degree of PhD at the
University of Groningen
on the authority of the
Rector Magnificus Prof. C. Wijmenga
and in accordance with
the decision by the College of Deans.
This thesis will be defended in public on
Friday 16 October 2020 at 12.45 p.m
by
Aline Lucie Odette Gaenssle
born on 20 April 1990
in Vienna, Austria
Supervisor
Prof. M.J.E.C. van der Maarel
Co-supervisors
Dr. E. Jurak
Dr. R.J. Leemhuis
Assessment committee
Prof. C. D'Hulst
Prof. M.W. Fraaije
Prof. H.A. Schols
Table of contents
Chapter 1
General introduction...7Chapter 2
Reliability factor for identification of low activity of amylolytic enzymes in the starch-iodine assay...45Chapter 3
The degree of branching of amylopectin is positively correlated to gelatinized starch digestibility...65Chapter 4
The structure of the final product of glycogen branching enzymes will be achieved irrespective of the substrate structure...85Chapter 5
GH13 Glycogen branching enzymes can adapt the substrate chain length towards their preferences via α-1,4-transglycosylation...107Chapter 6
Summary, discussion and conclusion...127Samenvatting...143
Acknowledgments...147