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Additional file 6. Oligonucleotides and restriction fragments used for preparation of probes. Probe

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Additional file 6. Oligonucleotides and restriction fragments used for preparation of probes.

Probe

PCR primers and restriction fragments

Template

HAC1

627 ScHAC1 fwd-1 CCCACCTACGACAACAACCGCCACT

628 ScHAC1 rev+660 CCTATGGATTACGCCAATTGTCAAG

pMS109 [11]

KAR2

629 ScKAR2fwd+212 AGACTGAAATTCTTGCTAATGAGC

630 ScKAR2rev+596 GCGTCATTGAAATAAGCAGGAAC

S. cerevisiae

PDI1

631 ScPDI1fwd+500 TAAGATTGACGCCGACTTCAAC

632 ScPDI1rev+900 TTGAGGCAAACCGTACTTCAAG

S. cerevisiae

ACT1

586 ACT1+50F ATGTGTAAAGCCGGTTTTGCC

587 ACT1+500R AGCGGTTTGCATTTCTTGTTC

S. cerevisiae

CBM+ENO

399Trcbh1 synt CBM5 MlyIHincII

GCGACGAGTCAACCCTCCAGGTGGTAACAGAGGTACTACCAC 410 ScENO1 term rev GAGCGGGCGGATACACGCGTC

pMI529 (this work)

T.e.cbh1

447 bp AgeI-BstEII fragment

pRDH105 (this work)

T.r.cbh2

418 bp MscI-XcmI fragment

pRDH107 (this work)

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