University of Groningen
Novel fluorescent probes and analysis methods for single-molecule and single-cell
microscopy
Smit, Jochem
DOI:10.33612/diss.102269758
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Publication date: 2019
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Smit, J. (2019). Novel fluorescent probes and analysis methods for single-molecule and single-cell microscopy. Rijksuniversiteit Groningen. https://doi.org/10.33612/diss.102269758
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Acknowledgements
First, I would like to acknowledge my supervisorsThorben Cordes and Andreas
Her-rmann. Thank you for the many years working together, teaching me everything about
chemistry and microscopy and your unparalleled support during my PhD. We had many memorable and exciting meetings together with several publications as a result.
Antoine van Oijen, thanks for welcoming us in your lab, your continuous support
over the years, and for creating a friendly and stimulating atmosphere where everyone enjoyed doing science.
In addition, I would like to thank Tassos Economou for your input and insightful suggestions and helping me to finalize this thesis.
My paranymphs; Ruud, we both started out on our bachelors together and if you hadn’t been studying physics I might not have been convinced to do so as well and I would have missed out on all of those exciting experiments and derivations. We spend many hours together doing homework and debating our favourite parts of physics. Aside from science, we played the occasional video game and sometimes had a beer together, or were hanging out with friends, which made studying a real pleasure. Jasper vdV, we studied together during our TOP master in Nanoscience, again, working on our homework and then continuing to work together for our master’s research and PhD. I remember a lot of fun times in the lab, just outside of Nb4 and sometimes we even made it to the city centre. Thanks for being my partner-in-crime during all those years and for always having my back.
My favourite collaborator Eliza, thank you for making all the compounds I could wish for (and many more than that). You were always keeping me motivated to do more measurements and try out new things, and the many coffee breaks always gave us new insights.
Yichen, we’ve worked together for many years and during this time we figured out
how to best get our cells on the microscope and how to convince it to keep them in focus. Thank you for all the measurements you provided and the discussions we had on
how to best analyse them.
I also had the great pleasure of working with several students and collaborators;
Jingyi, Jan-Willem, Vanessa, Si, LJ, Martin, thank you for your help and
contribu-tions. Many of your compounds and data were used in this thesis.
I’d like to recognize the effort of the members of my reading committee; M.
Bramkamp, D.J. Scheffers and A.M. van oijen.
Randy, thank you for the artwork you’ve created for me, including the amazing
cover of this thesis, and the many barbeques you’ve organized.
I very much appreciate the assistance and support of Jeanette and Margriet for helping me deal with paperwork and organisational issues.
Completing this work would have been all the more difficult were it not for the support and friendship provided by the members of the different labs; PCBE: Mark,
Deepak, Minseok, Agnieszka, Enzymology: Paul, Wojtek, Łukasz; SMB: Jan-Peter, Karl, Victor C., Alex, Hylkje, Da, Victor K., Sameneh, Jason, Joris, Jelle, Andrew, Stepahnie, Guus, Michiel, Sarah, Lisanne, Enrico, Harshad, Flynn, Anne-bart; MBP: Pedro, Yuzhen, Ignacio, Melissa, Guus; MM: Marijn, Monique, Evelyn, Atieh, Florence, Kostas, Giorgos, Nikos, Yusran, and my new lab LMB: Lily, Athina, Srinath, Dries, Mohammed, Bindu, Jozefien, Maria, Luit, Ruixue, Jeroen, Sonja.
And finally, the biggest support of all, I’d like to thank my family and friends. You’ve simulated and nurtured my interest in science and your support has been continuous and unwavering throughout my PhD.
