Peptide profiling by capillary separation techniques coupled to mass
spectrometry
Gaspari, Marco
Citation
Gaspari, M. (2006, December 13). Peptide profiling by capillary separation techniques coupled to mass spectrometry. Retrieved from https://hdl.handle.net/1887/5431
Version: Corrected Publisher’s Version
Curriculum Vitae
Marco Gaspari was born in Cosenza, on October 12th, 1971. In 1989 he completed high school at Liceo Scientifico Fermi, Cosenza. The same year he started studying chemistry at the University of Calabria. By joining the Erasmus Programme, he spent the academic year 1992/1993 at the University of Kent (Canterbury, UK), where he obtained the Diploma in Chemistry. The following year, at the University of Calabria, he joined the Laboratory of Organic Chemistry headed by Prof. Giovanni Sindona. He was awarded the Master Degree in Chemistry in 1995 with a thesis entitled “Synthesis of modified dinucleotides with phosphorothioate bridges”. After serving the Italian Army as lieutenant in the Technical Corps, he returned to University of Calabria to continue experimental work in organic chemistry. In 1998, he joined the Bio-Pharmaceutical analysis group at TNO Nutrition and Food Research (now TNO Quality of Life), Zeist, The Netherlands, where he spent six months as stagiere working on capillary electrophoresis and mass spectrometry of nucleic acids. The following year, 1999, he applied for a five-year position as Ph.D student/ part-time employee at the same department. The supervision of the Ph.D. thesis was entrusted to Prof. dr. Jan van der Greef, Head of the Department of Analytical Chemistry (now Analytical Biosciences), Leiden/Amsterdam Center for Drug Research (LACDR). The research presented in this thesis was almost entirely performed at the Bio-Pharmaceutical Department of TNO Nutrition and Food Research.
Since 2004 he holds a research grant (assegno di ricerca) thanks to which he has joined, under the supervision of Prof. Giovanni Cuda, the Proteomics/Mass Spectrometry Laboratory at the Department of Clinical and Experimental Medicine of University of Catanzaro “Magna Graecia”.
Acknowledgement
It is surely difficult to mention all the people who, directly or indirectly, contributed to the realization of this thesis.
First of all, I would like to acknowledge Sonja Jespersen for giving me many insights into biological mass spectrometry and for introducing me to proteomics research. Sonja has also often supervised my activities concerning projects outside my PhD research, always putting me in the conditions to give my best. Together with Sonja, in the proteomics team, I was lucky enough to work side by side with Kitty Verhoeckx. Apart for being an excellent collegue, she also become a great friend. It was fun and always very easy to work together whenever our projects were coming closer, and I had a lot of support in the difficult periods. I also greatly appreciate her friendship outside work, and I hope to have her and her family back again in Calabria for a holiday soon!
I’m very grateful to the many collegues at TNO who have always kindly supported and helped me, especially at the beginning, and have always maintained a cheerful atmosphere at work. I would like to mention especially the “historical” MS-group: Jaques, Laurence, Dick, Frans, Guus, Peter, Harm, Frank, Hugo, Inge, Ria, Henk, Bertram, Gerwin, Raymond and Lars. Furthermore, I’m especially grateful to Brigitte Buscher for introducing me to the world of capillary electrophoresis and for her
gastvrijheid towards me and Marjan when we first arrived to The Netherlands. A
special thought goes also to the pattern recognition group. In particular, I acknowledge Albert Tas, Jack Vogels, Florjan Wülfert, and Sabina Bijlsma for introducing to me concepts essential for the completion of my work. Another TNO group which I would like to mention is the protein research group, and especially Pim Nieuwhuizen, Sander Piersma and Stef Koppelman with whom I had interesting small collaborations through the years. I also would like to acknowledge Rob Stierum and Ben van Ommen for promoting and being part of the first proteomics project undertaken at TNO, and
Richard Rodenburg, with whom I had many occasions to talk about proteomics since he was one of Kitty’s supervisors. Finally, I’m very grateful to Michel de Reijer for giving me great freedom and support during the last year of my PhD research.
For the joint projects, I would like to acknowledge the two other members of the CEC-team, Marjan Guček and Karin Walhagen, for putting all their energy and enthusiasm. Marjan was also the first person I met when I arrived to TNO in 1998. We were both
stagiares for a 6-month period at that time. It was fun visiting places together, riding
to Utrecht with really old bikes and exchanging our first impressions on dutch lifestyle! For the CEC-MS project, I would also like to acknowledge Rob Vreeken for supervisioning our work and for the many stimulating discussions. Rob has also been a great teacher and reference point in LC-MS, and a person who I surely missed when he moved away from TNO a couple of years after I started my PhD research.
For the GST acetylation project, I would like to acknowledge Prof. Raj and Ekta Kohli. Ekta was a student of Prof. Raj at that time, and it was really pleasant to have her in the Netherlands for one week, working together at TNO, and hearing many stories about far away India.
I also enjoyed interesting collaboration with two other former PhD students at Leiden University: Anne Chartogne, who I appreciated for her spontaneity and for putting great care in performing and presenting her experimental work and Denise Jacobs, with whom I explored the difficult world of proteomics of species poorly represented in protein databases. I also appreciated very much Denise’s great trust (I hope justified) in my mass spec abilities, and her even greater trust (totally unjustified) in my dutch pronunciation, when she allowed me to read a “stelling” as paranimph at her Promotie. I also would like to acknowledge the entire group of Analytical Biosciences at Leiden University for the open and friendly atmosphere. I’m especially grateful to Bertil Hofte and Bea Reeuwijk for great support in the lab, and to Loes Beijersbergen for being such a nice and friendly presence in so many organizative matters .
List of publications
1. M. Guček, M. Gaspari, K. Walhagen, R.J. Vreeken, E.R. Verheij, J. van der Greef Capillary electrochromatography/nanoelectrospray mass spectrometry for attomole characterization of peptides.
Rapid Comm. Mass Spectrom. 2000, 14(16), 1448-1454.
2. K. Walhagen, M. Gaspari, U.R. Tjaden, G.P. Rozing, J. van der Greef
In-line coupling of low-pressure short capillary electrochromatography columns to electrospray ionisation mass spectrometry.
Rapid Comm. Mass Spectrom. 2001, 15(11), 878-883.
3. M. Gaspari, K. Walhagen, M. Guček, R.J. Vreeken, E.R. Verheij, J. van der Greef
Ion trap as detector for electrochromatography of peptides: possibilities and limitations.
J. Microc. Sep. 2001, 13(6), 243-249.
4. M. Gaspari, J. Vogels, F. Wulfert, A. Tas, K. Venema, S. Bijlsma, R.Vreeken, J. van der Greef
Novel Strategies in Mass Spectrometric Data Handling. Advances in Mass Spectrometry 2001, 15, 283-296.
5. A. Chartogne, M. Gaspari, S. Jespersen, B. Buscher, E. Verheij, R. van der Heijden, U. Tjaden, J. van der Greef
On-target fraction collection for the off-line coupling of capillary isoelectric focusing with matrix assisted laser desorption/ionisation mass spectrometry. Rapid Comm. Mass Spectrom. 2002, 16(3), 201-207.
6. E. Kohli, M. Gaspari, H.G. Raj, V.S. Parmar, J. van der Greef, G. Gupt, A.C. Watterson, C.E. Olsen
Establishment of the enzymatic protein acetylation in dependent of acetyl CoA: recombinant glutathione S-transferase 3-3 is acetylated by a novel membrane-bound transacetylase using 7,8-diacetoxy-4-methyl coumarin as the acetyl donor.
FEBS Lett. 2002, 530(1-3), 139-142.
7. R. Stierum, M. Gaspari, Y. Dommels, T. Ouatas, H. Pluk, S. Jespersen, J. Vogels, K. Verhoeckx, J. Groten, B. van Ommen
Proteome analysis reveals novel proteins associated with proliferation and differentiation of the colorectal cancer cell line Caco-2.
Biochim Biophys Acta-Proteins and Proteomics 2003,1650(1-2), 73-91.
8. E. Kohli, M. Gaspari, H.G. Raj, V.S. Parmar, S.K. Sharma, J. van der Greef, R. Kumari, G. Gupta Seema, P. Khurana, Y.K. Tyagi, A.C. Watterson, C.E. Olsen Acetoxy drug: protein transacetylase of buffalo liver-characterization and mass spectrometry of the acetylated protein product.
Biochim Biophys Acta- Proteins and Proteomics 2004, 1698(1), 55-66. 9. S.J. Koppelman, W.F. Nieuwenhuizen, M. Gaspari, L.M. Knippels, A.H.
Penninks, E.F. Knol, S.L. Hefle, H. de Jong
Reversible Denaturation of Brazil Nut 2S Albumin (Ber e1) and Implication of Structural Destabilization on Digestion by Pepsin.
J Agric Food Chem. 2005, 53(1), 123-131.
10. D.I. Jacobs, M. Gaspari, J. van der Greef, R. van der Heijden, R. Verpoorte Proteome analysis of the medicinal plant Catharanthus roseus.
Planta 2005, 221(5):690-704.
11. K. Verhoeckx, M. Gaspari, S. Bijlsma, R.P. Doornbos, J. van der Greef, R.F. Witkamp, R.J.T. Rodenburg
In search of secreted protein biomarkers for the anti-inflammatory effect of beta2-adrenergic receptor agonists: application of DIGE technology in combination with multivariate and univariate data analysis tools. Journal of Proteome Research 2005, 4(6), 2015-2023.
12. S.R. Piersma, M. Gaspari, S.L. Hefle, S.J. Koppelman Proteolytic processing of the peanut allergen Ara h 3. Mol Nutr Food Res. 2005, 49(8), 744-755.
13. M. Gaspari, K.C. Verhoeckx, E.R. Verheij, J. van der Greef
Integration of two-dimensional LC-MS with multivariate statistics for comparative analysis of proteomic samples.
