Cover Page The handle http://hdl.handle.net/1887/47927

Using isotype-specific antibodies, we could show that ACPA-expressing B cells in peripheral blood are mostly class-switched memory B cells expressing IgG, but also IgA, IgM and

Nevertheless, it has been postulated that these high ACPA serum titres in RA patients are due to a persistently active immune response against citrullinated proteins, as suggested

This secretion of ACPA-IgG was maintained even if the CD20 + B cell compartment had been depleted prior to Figure 3.3: Spontaneous anti-citrullinated protein antibodies

CD19 + live B cells were considered specific for citrullinated antigens if they stained positive for both CCP2 tetramers and negative for the arginine (CArgP2) control variant..

Aim: To obtain insight into the characteristics and the development of human autoreactive B cells in the context of rheumatoid arthritis (RA), we studied the expression of

Methods: B cells and plasmablasts/-cells obtained from freshly isolated peripheral blood and synovial fluid mononuclear cells (PBMC/SFMC) of RA patients were stained

Both type of experiments help to understand the citrullinated antigen-specific immune response and could lead towards new therapeutic targets for ACPA (B cell) specific

Daarom hebben wij gekeken of CD28 op de B-cellen uit het bloed en uit het synoviaal vocht van RA patiënten tot expressie wordt gebracht en of de cellen die ACPA tot expressie