University of Groningen
Ultrasound-triggered release and activation of drugs and biomacromolecules from nucleic acid scaffolds
Zhao, Pengkun
DOI:
10.33612/diss.168542653
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Publication date: 2021
Link to publication in University of Groningen/UMCG research database
Citation for published version (APA):
Zhao, P. (2021). Ultrasound-triggered release and activation of drugs and biomacromolecules from nucleic acid scaffolds. University of Groningen. https://doi.org/10.33612/diss.168542653
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Ultrasound-triggered Release and Activation of
Drugs and Biomacromolecules from
Nucleic Acid Scaffolds
Ultrasound-triggered Release and Activation of Drugs and Biomacromolecules from
Nucleic Acid Scaffolds
Pengkun Zhao
PhD thesis
University of Groningen
Zernike Institute PhD thesis series 2021-11
ISSN 1570-1530
The research described in this thesis was performed in Polymer Chemistry and
Bioengineering group at Zernike Institute for Advanced Materials, University of Groningen,
the Netherlands. This work was financially supported by the China Scholarship Council
(CSC), the University of Groningen, the Netherlands Organization for Science Research
(NWO) and European Research Council (ERC).
Cover design: Pengkun Zhao
Ultrasound-triggered Release and
Activation of Drugs and
Biomacromolecules from
Nucleic Acid Scaffolds
PhD thesis
to obtain the degree of PhD at the University of Groningen
on the authority of the
Rector Magnificus Prof. C. Wijmenga and in accordance with
the decision by the College of Deans. This thesis will be defended in public on
Friday 7 May 2021 at 14.30 hours
by
Pengkun Zhao
born on 21 December 1990 in Henan, China
Supervisor
Prof. A. HerrmannCo-supervisor
Prof. G. PortaleAssessment Committee
Prof. R.Sijbesma Prof. C. Bolm Prof. G. RoelfesContents
1. Remotely triggered release and activation of drugs from nucleic
acid-mediated platforms... 3
1.1 Introduction ... 4
1.2 Electric filed-triggered release ... 5
1.2.1 Electroporation-induced release ... 6
1.2.2 Redox reaction induced release ... 7
1.3 Magnetic force-triggered release ... 8
1.3.1 Magnetic guidance ... 8
1.3.2 Magnetic hyperthermia induced release ... 9
1.4 Light-triggered release ... 10 1.4.1 Photothermal activation ... 11 1.4.2 Photochemical activation ... 12 1.4.3 Photoisomerization activation ... 15 1.5 Ultrasound-triggered release ... 17 1.5.1 Sonoporation ... 17 1.5.2 Cavitation ... 18 1.5.3 Phase transition ... 19
1.6 Multiple stimuli-triggered release ... 19
1.7 Conclusion ... 21
1.8 Thesis motivation and overview ... 22
References ... 26
2. Ultrasound promoted drug activation of aminoglycoside antibiotics
from poly-aptamers ... 31
Abstract ... 32
2.1 Introduction ... 33
2.2 Results and discussion ... 35
2.3 Conclusions ... 40
2.4 Experimental sections ... 40
3. Activation of the catalytic activity of thrombin by ultrasound ... 53
Abstract ... 54
3.1 Introduction ... 55
3.2 Results and discussion ... 57
3.3 Conclusions ... 62
3.4 Experimental sections ... 62
References ... 72
4. Force-controlled self-assembly of nanoparticles and its application
for protein activation ... 77
Abstract ... 78
4.1 Introduction ... 79
4.2 Results and discussion ... 80
4.3 Conclusions ... 84
4.4 Experimental sections ... 85
References ... 91
5. Colorimetric assay to detect interactions between proteins and
nucleic acids ... 93
Abstract ... 94
5.1 Introduction ... 95
5.2 Results and discussion ... 97
5.3 Conclusions ... 102 5.4 Experimental sections ... 102 References ... 108
