Cover Page
The handle http://hdl.handle.net/1887/66032 holds various files of this Leiden University
dissertation.
Author: Fleurbaaij, F.
Title: Novel applications of mass spectrometry-based proteomics in clinical microbiology
Issue Date: 2018-09-27
Novel applicatioNs of
mass spectrometry-based
proteomics iN cliNical
microbiology
Frank Fleurbaaij
ISBN: 978-94-930-1982-9
Cover and layout: Bregje Jaspers, ProefschriftOntwerp.nl Print: ProefschriftMaken, ProefschriftMaken.nl
©2018 Frank Fleurbaaij. All rights reserved. No part of this book may be reproduced, stored in a retrieval system or transmitted in any form or by any means without permission of the author or the journals holding the copyrights of the published manuscripts.
This work was supported financially supported by The Netherlands organisation of scientific research (NWO, ZonMW grant number 50-51700-98-142).
Novel applicatioNs of
mass spectrometry-based
proteomics iN cliNical
microbiology
Proefschrift
ter verkrijging van
de graad van Doctor aan de Universiteit Leiden, op gezag van Rector Magnificus prof.mr. C.J.J.M. Stolker,
volgens besluit van het College voor Promoties te verdedigen op donderdag 27 september 2018
klokke 13:45 uur
door Frank Fleurbaaij geboren 9 november 1988
te Amsterdam
Promotor
Prof. Dr. E. J. Kuijper Co-promotoren Dr. H. C. van Leeuwen Dr. P. J. Hensbergen
Leden promotiecommissie Prof. Dr. M. Wuhrer
Prof. Dr. A. van Belkum Prof. Dr. C. R. Jiminez Prof. Dr. J. T. van Dissel
“Success does not consist in never making blunders but
in never making the same one a second time.”
- Henry Wheeler Shaw, 1874
Table of contents
Chapter 1 Introduction
Chapter 2 Capillary-electrophoresis mass spectrometry for the detection of carbapenemases in (multi-)drug-resistant Gram-negative bacteria
Chapter 3 Direct detection of extended-spectrum beta-lactamases (CTX-M) from blood cultures by LC-MS/MS bottom-up proteomics
Chapter 4 Typing Pseudomonas aeruginosa isolates with ultrahigh resolution MALDI-FTICR mass spectrometry
Chapter 5 Proteomic identification of Axc, a novel beta-lactamase with carbapenemase activity in a meropenem-resistant clinical isolate of Achromobacter xylosoxidans
Chapter 6 Summary and discussion Chapter 7 Nederlandse samenvatting List of publications
Curriculum vitae
Acknowledgements
9 37
55 71 93
119 135 141 143 145
