University of Groningen The oligomeric protein interference assay method for validation of antimalarial targets de Assis Batista, Fernando

University of Groningen

The oligomeric protein interference assay method for validation of antimalarial targets

de Assis Batista, Fernando

DOI:

10.33612/diss.94898872

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Publication date: 2019

Link to publication in University of Groningen/UMCG research database

Citation for published version (APA):

de Assis Batista, F. (2019). The oligomeric protein interference assay method for validation of antimalarial targets. University of Groningen. https://doi.org/10.33612/diss.94898872

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Propositions

accompanying the thesis

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Fernando A. B

1. The significant limitations found in the current antimalarial target validation tool set highlight the need for novel specific validation techniques (this thesis). 2. Oligomeric interface disruption can be achieved by the introduction of mutant

subunits in oligomeric protein complexes (this thesis).

3. Manipulation of oligomeric surfaces allow for control of specific enzyme activity in vitro and in vivo (this thesis).

4. Oligomeric surfaces possess a high potential to be explored in the validation of essential oligomeric enzymes of Plasmodium parasites (this thesis).

5. The oligomeric protein interference assay (PIA) is a minimally perturbing method to specifically inhibit metabolic pathways of interest with a minimal off-target effect (this thesis).

6. Enzymes integral to the aspartate biosynthetic pathway of Plasmodium falciparum are promising targets for the discovery of novel antimalarials (this thesis).

7. Learning how to keep the excitement in frustration might be the most important skill you will learn during your PhD (my view).

8. Ideas are like rabbits. You get a couple and learn how to handle them, and pretty soon you have a dozen (John Steinbeck).

9. Succes is soms het resultaat van een hele reeks mislukkingen (Vincent van Gogh).