University of Groningen
Probing the ligand receptor interface of TNF ligand family members RANKL and TRAIL Wang, Yizhou
DOI:
10.33612/diss.127959201
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Publication date: 2020
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Wang, Y. (2020). Probing the ligand receptor interface of TNF ligand family members RANKL and TRAIL. University of Groningen. https://doi.org/10.33612/diss.127959201
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Appendices
Acknowledgements
List of publications
About the author
Achknowledgements
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Acknowledgements
Today is my 30th birthday and it is a great opportunity to recall my PhD life in the Netherlands. In the last four and a half years, I have been so lucky to meet all of you and work in such a supportive environment. At this moment, I would like to express my gratitude to everyone who helped me with this journey.
First of all, I would like to express my deep thanks to my supervisor Prof. dr. Wim. J. Quax. Dear Wim, thank you for offering me the opportunity to study under your supervision and for supporting me through these years. I have learned a lot from your guidance, not only the broad knowledge but also scientific thinking and problem-solving skills. I cherish every opportunity to have the meeting with you because you can always solve problems that have troubled me for a long time, and you always cheer me up when I feel worried during my study. Besides, you encourage me to cooperate with other departments and support me to join the international conference, which allowed me to learn the frontiers of research on cell death. Apart from work, you are also very kind and patient to everyone. I appreciate your care for the ―elevator people‖ and your organization for the department during the Covid-19 lockdown. I wish you all the best.
I would like to thank my second supervisor Prof. dr. Hidde. J. Haisma. Dear Hidde, we have had frequent communications since Olivia and I started the project on adenoviral-expressed RANKL WT/ Q236D. I enjoy our discussions at every meeting, and I learned a lot of knowledge about adenovirus from you. Special thanks to you for your patience and time to help me revise my thesis in the end period of my PhD. My sincere thanks to Prof. dr. Gerrit
J. Poelarends and Prof. dr. Frank J. Dekker for the kind comments and constructive
suggestions for my PhD projects.
I would like to thank the members of my assessment committee: Prof. R.A. Bank, Prof. B.N.
Melgert, and Prof. V.W. van Beusechem for taking the time to evaluate and comment on my
thesis.
I would also like to thank Dr. Marco Demaria and Prof. Barbro. N. Melgert for helping me with my research projects. Dear Marco, thank you for the support and discussions on the ―TRAIL-senescent‖ story, from which I got a huge interest in the field of cellular senescence. Dear Barbro, thank you for the guidance on my RANKL project. In the meantime, I would also like to show my gratitude to you for the knowledge of statistics analysis.
169 Dear Dr. Robbert. Cool, I don't know how to express my gratitude to you because you have given me too much help and support along the way in the past four and a half years. Thanks to you, I systematically learned protein-related knowledge and technologies. You spent a lot of time and effort teaching me to use the AKTA and Biacore machines. I destroyed the Biacore a thousand times, but you are always patient to help me ―kick off the devils.‖ Thanks for translating the Dutch Summary for my thesis and helping me write the recommendation letter. We have a lot of communications both at and out of the work. I appreciate your rigorous attitude towards scientific research and your optimism towards life. I will move forward with your encouragement and support.
Dear Ykelien, thank you for being my second supervisor in the first two years of my PhD. You gave me a lot of guidance on my projects and taught me the techniques to be a strong/confident woman. Thank you for inviting me to dinners with different interesting topics and for the best desserts in the world, especially the matcha ice-cream. Importantly, thank you for the daily hugs, which are the warmest gifts you gave to me.
Dear Rita, thank you for everything! You are always generous and ready to help others. Without you, I definitely could not finish this thesis smoothly. I feel lucky to meet you in a foreign country and I seem to be used to sharing my emotions with you. The conference trip to Dresden made us know each other better. I appreciate your honesty and bravery. Love you because you are unique Rita! Dear Ronald, thank you for introducing the Discovery studio software and some advanced/effective techniques to me. Thank you for always helping me to solve various problems efficiently. I enjoy our discussions about selecting RANKL and TRAIL mutants, although sometimes I am stubborn. Dear Pieter, thank you for helping me with ordering reagents and arranging lab issues. Thank you for your concern after the elevator accident. Dear Petra, thank you for your help and quick response when I came to you. I wish you all the best!
Dear Yvonne, Janita, and Gillian, thank you for your professional work, which allows me to conduct PhD study efficiently. I would also like to thank Anne and Cathy for helping me with PhD registration and extension related issues.
My dear officemates, Brenda, Magda, Abel, and Xinyu. We spent these years together in perfect harmony. I am very lucky to meet each of you. Brenda, we accompanied each other through many unforgettable moments. Thank you, my 小师姐，for your continuous support and encouragement to me. I admire your bravery and very happy for you to get a postdoc
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position in Dublin. I wish you, Louis, and Xiaodong all the best. Magda, this world may sometimes be unfriendly, but the sunshine always comes after the rain. I appreciate your versatility and your passion for life. Thank you for the various interesting conversations, for the dinners and the living room concerts. You are amazing. I wish you all the best in Gdansk.
Abel, my paranymph, I feel very lucky to have a close friend like you in my life. You are so
smart with broad knowledge. You have the most beautiful heart, always thinking about others. I believe we will keep in touch! Xinyu, I enjoy chatting with you a lot. Thank you for so many smart tips both at and out of work. I wish your PhD study goes smoothly. I love you all! My sincere gratitude to people who also help me with my research projects. Abel, it is so pleasant to work with you. Thank you for your patience and focus. Olivia, you are very kind and sweet. Thank you for the adenovirus related knowledge and techniques. I wish you a bright future. Patiwat, as an exchange student, although you have limited time to work here, it is very helpful for you for the progress of the TRAIL mutants project. I wish you all the best. Fengzhi, I am glad you could continue with the RANKL work. Good luck with the animal experiments! Habibie, we have had a lot of interesting discussions on each other‘s projects. We also provide relevant experimental materials to each other. I wish you good luck with your PhD and your family all the best. Besides, I would like to thank Bin and Lin for the discussions and support. Thank Catherina, Eduard, and Michela (ERIBA) for their guidance and assistance in Real-time quantitative PCR measurement. Of course, I would like to thank my lovely students, Timo, Amy, Naira, Federico, Lorina, Nora, and Maysam. I wish you all have a bright future!
My dear previous and current colleagues, thank you for creating an open, friendly and pleasant atmosphere in the department. Jielin, it is very funny that we came from the same hometown and university, but we started to know each other in Groningen. You are calm and thoughtful, and you always cheer me up when I am down. It‘s comfortable to stay with you and it was a wonderful trip with you in Portugal. I will always be your fan. Bin, Hao and
Haigen, we started the PhD study on the same day in the department. Thank you for taking
care of me over the years! Putri, thank you for your sweet and warm heart. I always feel cozy and relaxed with you. I wish you and Johannes a happy life! Yafeng, you are smart and hardworking, I am sure you will have a bright future. Thank you for your company in the past two years, which is my precious memory. Guangcai and Fangyuan, we used to have a study team with Yafeng and I miss the days that we work together. I wish you two a happy and sweet life! Siqi and Fengzhi, thank you for bringing me a lot of joy in the last period of my
171 PhD. It was full of fun to gather with you girls! Ingy, thank you for sharing your experience of constructing the mutation library, and thanks for the delicious Egyptian food! Ele and
Marie, it was pleasant to talk with you girls! Deng and Siwei, you are both doing excellent
jobs. Good luck! Jan, thank you for arranging the group meetings and bringing a lot of joy to the department. Joko, I wish you success in your PhD study. It's almost there! My sincere thanks to Ingrid, Christel, Jane, Linda, Hegar, Tjie, Dan, Yan, Lieuwe, Alex, Saif,
Saravanan, Zainal, Chao, Andreas, Michele, Laura, Shanshan, Fabiola, Hannah, Thea, Martijn, Zhangping, and Roberta, for your help and support in the department. I enjoyed
the time with you and I wish you all the best! For the new coming people, Kristina, Sandy and Michael, I wish you a great time in the department!
Special thanks to my friends for their support and accompany outside the working life. Lin, my paranymph, you are gorgeous with forthright and sincerity. Thanks for being such strong support for me during the last period of my PhD. Bin, thank you for a lot of interesting conversations and for choosing the right foundation color for me. I wish you, Lin and
Mengmeng an early reunion. Yana, this is the 13th year we have known each other, but it seems that I just started to know you in the past recent year. I miss those nights when we talked (and drank) until we lost track of time. See you in China! Keni, you are the best! Life is like a circle, the same as our friendship. Hao and Yu, thank you for your care in the last several years. I am sure we will keep in touch in the future. Shanshan, thank you for a lot of good memories. I wish you and Zhangping a happy life. Yehan, Wenxuan and Huala, I feel lucky to meet you girls! Su, you are intellectual and beautiful. I wish you, Naqin and your lovely baby a happy future! I am truly thankful to my other friends, Haigen & Xiaoxuan,
Yang, Liwen, Xiaohong, Yuzhen, Mingming, Chengtao, Huatang, Zhenchen, Chengcheng, Chenxi, Lianghui, Wenjia, Xinhui, Kai, Chao, Qian, Jingyao , Xiaoxiang, Daozheng, Jingjing & Yuanze, Yuanyuan & Liqiang, Jiabao, Xiaoyin, Yingying & Changsen, Peng, Xin. It‘s so nice to meet you in Groningen!
Many thanks to my good friends far away in China, for their support and encouragement, even though we are a thousand miles away. They are Jing, Xuan, Xiaoting, Shuya, Xin
Wang, Luting, Kexin and Feiran.
I sincerely thank the Chinese Scholarship Council for offering me this opportunity to pursue my PhD study abroad. Thank my supervisors at the master‘s degree, Dr. Hui Wang and Prof.
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Special thanks to Yaoyao’s family. You made me feel at home in a foreign country. Yaoyao and Eason, Aunt Ning loves you forever!
My dear Qifeng, we have known each other for eight years and have been married for four years. Although it is often a long-distance relationship, I feel you are always by my side. Thank you for your unconditional tolerance, support and love. We will never be apart. Thanks a lot to my parents-in-law, for your kind support and warm regards during my stay in the Netherlands.
Dear Dad and Mom, I feel lucky to be your daughter. I love you!
亲爱的爸爸妈妈，很幸运成为你们的女儿，希望我们仨永远都走不散，我爱你们！
Yizhou 22-04-2020
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List of publications
1. Yizhou Wang, Timo Michiels, Rita Setroikromo, Ronald van Merkerk, Robbert H. Cool and Wim J. Quax#. (2019) Creation of RANKL mutants with low affinity for decoy receptor OPG and their potential anti-fibrosis activity. FEBS. J. 286(18): 3582-3593. 2. Yizhou Wang*, Aart H.G. van Assen*, Carlos R. Reis, Rita Setroikromo, Ronald van
Merkerk, Ykelien L. Boersma, Robbert H. Cool and Wim J. Quax#. (2017) Novel RANKL DE-loop mutants antagonize RANK-mediated osteoclastogenesis. FEBS. J. 284(15), 2501-2512.
3. Wim J. Quax#, Aart H.G. van Assen and Yizhou Wang. (2018) TNF-family member Receptor Activator of NF-κB (RANK) and RANK-Ligand (RANKL) in bone remodelling. IOP Conference Series: Earth and Environmental Science. IOP PUBLISHING LTD, 9 p. 012001.
4. Yizhou Wang*, Yikang Gu*, Kai Fang, Ke Mao, Jie Dou, Hongye Fan, Changlin Zhou and Hui Wang. (2018) Lactobacillus acidophilus and Clostridium butyricum ameliorate colitis in murine by strengthening the gut barrier function and decreasing inflammatory factors. Beneficial microbes. 9(5):775-787.
5. Mengyun Ke, Hui Wang, Min Zhang, Yuwei Tian, Yizhou Wang, Bing Li, Jie Yu, Jie Dou, Tao Xi and Changlin Zhou. (2014) The anti-lung cancer activity of SEP is mediated by the activation and cytotoxicity of NK cells via TLR2/4 in vivo. Biochem Pharmacol. 89 (1):119-30.
6. Hui Wang, Mengyun Ke, Yuwei Tian, Jing Wang, Bing Li, Yizhou Wang, Jie Dou and Changlin Zhou. (2013) BF-30 selectively inhibits melanoma cell proliferation via cytoplasmic membrane permeabilization and DNA-binding in vitro and in B16F10-bearing mice. Eur J Pharmacol, 707: 1-10.
Manuscripts in preparation
1. Yizhou Wang*, Olivia Adaly Diaz Arguello*, Rita Setroikromo, Fengzhi Suo, Hidde J. Haisma and Wim J. Quax#. Long term secretion of adenovirally-expressed RANKL WT/ Q236D for treatment of fibrosis. In preparation.
2. Abel Soto Gamez*, Yizhou Wang*, Lorina Seras, Rita Setroikromo, Marco Demaria and Wim J. Quax#. Overcoming senescent cancer cell resistance to extrinsic apoptosis using a death receptor 5 (DR5) selective TNF-related apoptosis inducing ligand (TRAIL) variant. In preparation.
3. Fengzhi Suo*, Yizhou Wang*, Patiwat Kongdang, Euphemia Amelia in het Veld, Rita Setroikromo, Ronald van Merkerk, Robbert H. Cool and Wim J. Quax#. Novel TRAIL variants with reduced binding to decoy receptor OPG overcome TRAIL resistance in breast cancers. In preparation.
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4. Yizhou Wang*, Bin Liu*, Deng Chen, Abel Soto Gamez, Rita Setroikromo, Hidde J. Haisma and Wim J. Quax#. Inhibition of CXCR7 sensitizes TRAIL-induced apoptosis in breast cancer cells. In preparation.
5. Bin Liu*, Deng Chen*, Jelle van den Bor, Yizhou Wang, Martine J. Smit, Frank J. Dekker, Hidde J. Haisma#. A novel interaction of EGF and CXCR7. In preparation.
6. Habibie, Shanshan Song, Kurnia S.S. Putri, Carian E Boorsma1, Robbert H Cool, Xinhui Wu, Reinoud Gossens, Yizhou Wang, Wim J Quax, Peter Olinga, Corry-Anke Brandsma, Wim Timens, Janette K Burgess, Barbro N Melgert#. RANKL contributes to lung tissue repair via promoting type II epithelial cell proliferation. In preparation.
Conferences
1. Chemistry as Innovating Science: The Dutch Chemistry Conference (Eindhoven, NL, Dec, 2016)
2. Chemistry as Innovating Science: The Dutch Chemistry Conference (Eindhoven, NL, Dec, 2017) Poster presentation: Novel RANKL DE-loop mutants antagonize RANK-mediated osteoclastogenesis.
3. Cell Death and Regeneration: The Conference of European Cell Death Organization (Dresden, Germany, Sep, 2019) Poster presentation: Creating structure-based RANKL mutants with low affinity for OPG to activate macrophage in fibrosis.
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About the author
Yizhou Wang was born on the 22nd of April 1990 in Hebei, China. In June 2012, she obtained her Bachelor‘s degree in Biotechnology from China Pharmaceutical University. In June 2015, she earned her Master‘s degree in Microbial and Biochemical Pharmacy in the same university under the supervision of Prof. Changlin Zhou. In October 2015, she moved to The Netherlands to pursue her doctoral degree in the Department of Chemical and Pharmaceutical Biology, University of Groningen, under the supervision of Prof. Wim. J. Quax. Her doctoral research focuses on probing the ligand-receptor interface of TNF ligand family members
