University of Groningen
Unravelling the mechanisms of recognition and internalization of nanoparticles by cells
Montizaan, Daphne
DOI:
10.33612/diss.136290962
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Publication date: 2020
Link to publication in University of Groningen/UMCG research database
Citation for published version (APA):
Montizaan, D. (2020). Unravelling the mechanisms of recognition and internalization of nanoparticles by cells. University of Groningen. https://doi.org/10.33612/diss.136290962
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Unravelling the mechanisms of
recognition and internalization
of nanoparticles by cells
Unravelling the mechanisms of recognition and
internalization of nanoparticles by cells
PhD thesis
to obtain the degree of PhD at the University of Groningen
on the authority of the Rector Magnificus Prof. C. Wijmenga
and in accordance with the decision by the College of Deans. This thesis will be defended in public on Friday 6 November 2020 at 11.00 hours
by
Daphne Montizaan born on 26 September 1992 in Geldrop, The Netherlands
The work described in this thesis was performed at the department Nanomedicine and Drug Targeting at the University of Groningen, The Netherlands. The research was funded by the European Research Council (ERC) as part of the European Union’s Horizon 2020 research and innovation program under the grant agreement no 637614 (NanoPaths).
The printing of this thesis was financially supported by the research institute GUIDE and the Graduate School of the University of Groningen.
Cover design: Melanie Montizaan and Jordi van Duijn Layout: proefschrift-aio
Printing: proefschrift-aio
© Copyright by Daphne Montizaan, Groningen, the Netherlands, 2020. All right reserved. No part of this thesis may be reproduced in any form or by any means without prior permission of the author.
Table of content
Chapter 1 Introduction: Interactions at the cell membrane and
pathways of internalization of nano-sized materials for nanomedicine
Scope and outline of the thesis
Chapter 2 Comparison of the uptake mechanisms of zwitterionic
and negatively charged liposomes by HeLa cells
Chapter 3 Preparation and cell uptake of liposome and
liposome-coated silica: effects of nanoparticle rigidity and role of curvature-sensing proteins
Chapter 4 Dynamic response of the pathways involved in
nanoparticle uptake in a genome-wide time-resolved forward genetic screening
Chapter 5 Novel insights by forward genetic screening into
nanoparticle uptake by cells: role of heparan sulphate and apolipoprotein receptors in the early interactions on the cell membrane
Chapter 6 General discussion and future perspectives
Nederlandse samenvatting Acknowledgements
About the author List of publications
Supervisors Prof. A. Salvati Prof. K. Poelstra
Assessment Committee Prof. A.T. Jones Prof. R.M. Schiffelers Prof. H.J. Haisma 7 42 47 79 119 163 211 228 244 248 249
