Towards a blood stage malaria vaccine, dealing with allelic
polymorphism in the vaccine candidate apical membrane antihen 1
Kusi, K.A.
Citation
Kusi, K. A. (2012, January 18). Towards a blood stage malaria vaccine, dealing with allelic polymorphism in the vaccine candidate apical membrane antihen 1. Retrieved from
https://hdl.handle.net/1887/18387
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License: Licence agreement concerning inclusion of doctoral thesis in the Institutional Repository of the University of Leiden
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Towards a blood stage malaria vaccine; dealing with allelic polymorphism in the vaccine
candidate apical membrane antigen 1
Proefschrift
ter verkrijging van de graad van
Doctor aan de Universiteit Leiden, op gezag van Rector Magnificus prof.mr. P.F. van der Heijden, volgens besluit van het College voor Promoties te
verdedigen op woensdag 18 januari 2012 klokke 15:00 uur
door
Kwadwo Asamoah Kusi
geboren te Accra, Ghana in 1977
Promotie commissie
Promotor Prof.dr. A.M. Deelder
Co-promotor Dr. C.H.M. Kocken, BPRC, Rijswijk
Co-promotor Dr. E.J. Remarque, BPRC, Rijswijk
Overige leden Prof. dr. R.W. Sauerwein, UMC St. Radboud, Nijmegen Dr. D. Dodoo, NMIMR, Ghana.
Prof.dr. R.R.P. de Vries Prof.dr. T.H.M. Ottenhoff Prof.dr. M. Yazdanbakhsh
The studies described in this thesis were performed at the Biomedical Primate Research Centre (BPRC) in Rijswijk, the Netherlands, with funds from EMVI, EMVDA and EUROMALVAC. The author was also supported financially by a GETFund scholarship (Ghana).
Printing of this thesis was with financial support from the GETFund, BPRC and BioGenes GmbH, Berlin.
Cover design: Henk van Westbroek
Printing: Off Page
ISBN: 978-94-6182-050-1
To Stella, the love of my life
and to Cheryl, the shining star in my life
Table of contents
Chapter 1 General introduction 1
Chapter 2 Humoral immune response to mixed PfAMA1 alleles; 27 multivalent PfAMA1 vaccines induce broad specificity.
Chapter 3 Generation of humoral immune responses to multi-allele 53 PfAMA1 vaccines; effect of adjuvant and number of
component alleles on the breadth of response.
Chapter 4 Immunisation with different PfAMA1 alleles in 77
sequence induces clonal imprint humoral responses that are similar to responses induced by the same alleles as a vaccine cocktail in rabbits. Chapter 5 Safety and immunogenicity of multi-antigen AMA1- 95
based vaccines formulated with CoVaccine HT™ and Montanide ISA 51 in rhesus macaques. Chapter 6 Measurement of the plasma levels of antibodies 115
against the polymorphic vaccine candidate apical membrane antigen 1 in a malaria-exposed population. Chapter 7 General discussion 131
Summary/Nederlandse samenvatting 143
List of publications 151
Acknowledgements 153
Curriculum vitae 155
Supplementary figures 157
