Synthetic vesicles for metabolic energy conservation

University of Groningen

Synthetic vesicles for metabolic energy conservation

Pols, Tjeerd

DOI:

10.33612/diss.143823522

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Publication date:

2020

Link to publication in University of Groningen/UMCG research database

Citation for published version (APA):

Pols, T. (2020). Synthetic vesicles for metabolic energy conservation. University of Groningen.

https://doi.org/10.33612/diss.143823522

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PROPOSITIONS

belonging to the PhD thesis

Synthetic vesicles for metabolic energy conservation

by

Tjeerd Pols

1. “Omnis cellula e cellula.” - Rudolf Virchow (1855)

2. The Lactococcus lactis IL1403 arginine/ornithine antiporter is relatively slow and presumably rate-determining for ATP production from arginine breakdown. - This thesis, Chapter 2

3. Reconstitution of a bacterial pathway inside lipid vesicles can lead to side reactions that may not occur in the bacterium.

- This thesis, Chapter 3

4. Coupling arginine breakdown to the glycine betaine importer OpuA allows the system to maintain a metabolic state far-from-equilibrium for many hours. - This thesis, Chapter 3

5. The importance of cellular homeostasis is often overlooked in studies on building of synthetic cells.

– This thesis, Chapter 4

6. “In general, quantum mechanics does not predict a single definite result for an observation. [...] Quantum mechanics therefore introduces an unavoidable element of unpredictability or randomness into science.”

– Stephen Hawking, A brief history of time (2016), p. 64

7. “Chemiosmotic coupling constrained the evolution of life on earth to the

complexity of bacteria and archaea for billions of years. A singular event, in which one bacterium somehow got inside another one, overcame these endless energetic constraints on bacteria.”

– Nick Lane, The vital question (2016), p. 86

8. “Many copies of good genes are dragged under because they happen to share a body with bad genes, and many perish through other forms of ill luck.” – Richard Dawkins, The selfish gene (2016), p. 49