Classification of cell populations in CTC enriched samples by advanced image analysis

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Classification of cell populations in CTC enriched samples by advanced image analysis

Sanne de Wit

1

_{, Leonie L. Zeune}

1,2

_{, Christoph Brune}

2

_{, Guus van Dalum}

3

_{, T. Jeroen N. Hiltermann}

4

_{, Harry J.M. Groen}

4

_{, Leon W.M.M. Terstappen}

1

1 _{Department of Medical Cell BioPhysics, University of Twente, the Netherlands,}2 _{Department of Applied Mathematics, University of Twente, the Netherlands,}3 _{Department of General, Visceral and}

Pediatric Surgery, University Hospital and Medical Faculty of the Heinrich-Heine-University, Germany, 4 _{Department of Pulmonology, University Medical Center Groningen, the Netherlands}

Abstract 3645

AACR 2018

Automated CTC Classification Enumeration and PhenoTyping

Deep Learning

CTC Scoring Consensus

Quantification of nucleated cells by gating for various parameters using CellSearch images.

For segmentation of fluorescent signal: Is there any signal present?

For classification of cell populations: Is this cell a CTC or not?

ACCEPT

A total of 100 cells were scored by an Expert Panel, 15 Reviewers and by Deep Learning Low agreement between reviewers: 37%

s.dewit@utwente.nl

CellSearch® cartridge for CTC enrichment

_{Classification and visualization of various cell populations by}

Cell Populations

gating for several parameters in ACCEPT.

CellSearch populations are improved by adding

CD16-PerCP to the immunostaining and using a LED light source, instead of a mercury arc lamp.

Improving Cell Classification

By using advanced image analysis of fluorescent images

obtained from EpCAM enriched blood samples, the complete cellular composition of the sample can be obtained.

Operator variability in classification of objects is eliminated as well as the time

spend by the operators to review the images.

Conclusion

Reviewers vs. Deep Learning 29% 51% 20% Expert Panel vs. Deep Learning 33% 40% 27% Reviewers vs. Expert Panel 33% 50% 17%

Agreement on a cell being “CTC” Agreement on a cell being “not a CTC” Disagreement