University of Groningen
Biochemical characterization of β-galactosidases and engineering of their product specificity
Yin, Huifang
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Publication date: 2017
Link to publication in University of Groningen/UMCG research database
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Yin, H. (2017). Biochemical characterization of β-galactosidases and engineering of their product specificity. University of Groningen.
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Biochemical Characterization of
β-Galactosidases and Engineering of Their
Product Specificity
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Printed by Ipskamp Printing, Enschede ISBN printed: 978-94-034-0083-9
ISBN digital: 978-94-034-0082-2
The work described in this thesis was carried out in the Microbial Physiology Group of the Groningen Biomolecular Sciences and Biotechnology Institute at the University of Groningen and was financially supported by the China Scholarship Council and the University of Groningen.
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Biochemical Characterization of
β-Galactosidases and Engineering of Their
Product Specificity
PhD thesis
to obtain the degree of PhD at the
University of Groningen
on the authority of the
Rector Magnificus Prof. E. Sterken
and in accordance with
the decision by the College of Deans.
This thesis will be defended in public on
Monday 25 September 2017 at 12.45 hours
by
Huifang Yin
born on 9 October 1987
in Shandong, China
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Supervisor
Prof. L. Dijkhuizen
Co-supervisor
Dr. S. S. van Leeuwen
Assessment Committee
Prof. D.B. Janssen
Prof. G.J. Boons
Prof. P. de Vos
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Contents
Chapter 1 β-Galactosidase enzymes and their galactooligosaccharide 7
products
Chapter 2 Reaction kinetics and galactooligosaccharide product profiles 29
of the β-galactosidases from Bacillus circulans, Kluyveromyces lactis and Aspergillus oryzae
Chapter 3 Engineering of the Bacillus circulans β-galactosidase 65
product specificity
Chapter 4 Biochemical characterization of the functional roles of 103
residues in the active site of the β-galactosidase from Bacillus circulans ATCC 31382
Chapter 5 Synthesis of oligosaccharides derived from lactulose by 131
wild-type and mutant β-galactosidase enzymes from
Bacillus circulans ATCC 31382 and their utilization
by Bifidobacteria
Chapter 6 Summary and perspectives 169
Samenvatting en vooruitzichten 179
ѝ᮷᪈㾱 191
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