The handle http://hdl.handle.net/1887/38545 holds various files of this Leiden University dissertation.
Author: Molendijk, Ilse
Title: Mesenchymal stromal cell therapy for Crohn's disease : from perianal fistulizing disease to experimental colitis
Issue Date: 2016-03-15
Mesenchymal stromal cell therapy for Crohn’s disease
from perianal fistulizing disease to experimental colitis
Ilse Molendijk
Cover design: Eveline de Jonge – Muller Cover photograph: Willem Vermaase
Printed by: Uitgeverij BOXPress || Proefschriftmaken.nl ISBN: 978-94-6295-452-6
© 2016, I. Molendijk, Leiden, the Netherlands
All rights reserved. No part of this thesis may be reproduced or transmitted in any form or by any means without prior written permission of the author.
The research described in this thesis was financially supported by a grant from the DigestScience Foundation.
Proefschrift
ter verkrijging van
de graad van Doctor aan de Universiteit Leiden, op gezag van Rector Magnificus prof. mr. C.J.J.M. Stolker,
volgens besluit van het College voor Promoties te verdedigen op dinsdag 15 maart 2016
klokke 15:00 uur door
Ilse Molendijk
geboren te Gouda in 1985 Financial support for the publication of this thesis was kindly provided by AbbVie B.V.,
ABN AMRO, BD Biosciences, ChipSoft B.V., Dr. Falk Pharma Benelux B.V., EuroTec B.V.,
Greiner Bio-One B.V., Dutch Society of Gastroenterology (NVGE), Olympus Nederland
B.V., Section Experimental Gastroenterology (SEG) of the Dutch Society of Gas-
troenterology (NVGE), Stöpler Instrumenten & Apparaten B.V., Takeda Nederland B.V.
Cover design: Eveline de Jonge – Muller Cover photograph: Willem Vermaase
Printed by: Uitgeverij BOXPress || Proefschriftmaken.nl
ISBN: 978-94-6295-452-6
© 2016, I. Molendijk, Leiden, the Netherlands
All rights reserved. No part of this thesis may be reproduced or transmitted in any form or by any means without prior written permission of the author.
Financial support for the publication of this thesis was kindly provided by
The research described in this thesis was financially supported by a grant from the DigestScience Foundation.
Mesenchymal stromal cell therapy for Crohn’s disease
from perianal fistulizing disease to experimental colitis
Proefschrift
ter verkrijging van
de graad van Doctor aan de Universiteit Leiden, op gezag van Rector Magnificus prof. mr. C.J.J.M. Stolker,
volgens besluit van het College voor Promoties te verdedigen op dinsdag 15 maart 2016
klokke 15:00 uur door
Ilse Molendijk
geboren te Gouda
in 1985
Prof. dr. W.E. Fibbe
Prof. dr. D.E. Atsma
Dr. B.A. Bonsing
Promotores Prof. dr. ir. H.W. Verspaget Prof. dr. D.W. Hommes
Co-promotor Mw. dr. A.E. van der Meulen – de Jong
Leden promotiecommissie Mw. prof. dr. C.J. van der Woude, Erasmus MC Rotterdam Prof. dr. G. Dijkstra, UMCG Groningen
Prof. dr. W.E. Fibbe
Prof. dr. D.E. Atsma
Dr. B.A. Bonsing
Chapter 2 Outline and aims of the studies described in this thesis 32
Chapter 3 Disappointing durable remission rates in complex Crohn’s 36 disease fistula
Inflammatory Bowel Diseases. 2014;20:2022-2028
Chapter 4 Allogeneic bone marrow-derived mesenchymal stromal 54 cells promote healing of refractory perianal fistulas in pa-
tients with Crohn’s disease
Gastroenterology. 2015;149:918-927Chapter 5 Standardization of local treatment for perianal fistulizing 80 Crohn’s disease
Submitted
Chapter 6 Time of administration and disease severity are key factors 98 in achieving efficacy of human bone marrow-derived mesen-
chymal stromal cells in an experimental model of colitis
Manuscript in preparationChapter 7 VCAM-dependent migration of IFNɣ- and TNFα-stimulated 120 mesenchymal stromal cells ameliorates TNBS-induced colitis
Manuscript in preparation
Chapter 8 Intraluminal injection of mesenchymal stromal cells in 146 spheroids attenuates experimental colitis
Submitted
Chapter 9 Summary and discussion 172
Dankwoord
List of abbreviations
T ABLE OF CONTENTS
Chapter 1 Introduction 8
Best Practice & Research: Clinical Gastroenterology. 2014;28:505-518, In: Mesenchymal stem cell therapy. 2013; Springer text book and J Allergy (Cairo). 2012;2012:187408
Chapter 2 Outline and aims of the studies described in this thesis 32
Chapter 3 Disappointing durable remission rates in complex Crohn’s 36 disease fistula
Inflammatory Bowel Diseases. 2014;20:2022-2028
Chapter 4 Allogeneic bone marrow-derived mesenchymal stromal 54 cells promote healing of refractory perianal fistulas in pa-
tients with Crohn’s disease
Gastroenterology. 2015;149:918-927Chapter 5 Standardization of local treatment for perianal fistulizing 80 Crohn’s disease
Submitted
Chapter 6 Time of administration and disease severity are key factors 98 in achieving efficacy of human bone marrow-derived mesen-
chymal stromal cells in an experimental model of colitis
Manuscript in preparationChapter 7 VCAM-dependent migration of IFNɣ- and TNFα-stimulated 120 mesenchymal stromal cells ameliorates TNBS-induced colitis
Manuscript in preparation
Chapter 8 Intraluminal injection of mesenchymal stromal cells in 146 spheroids attenuates experimental colitis
Submitted
