University of Groningen
Fabrication and Mechanical properties of Supercharged polypeptides based Biomaterials:
from Adhesives to Fibers
Sun, Jing
DOI:
10.33612/diss.116872472
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Publication date: 2020
Link to publication in University of Groningen/UMCG research database
Citation for published version (APA):
Sun, J. (2020). Fabrication and Mechanical properties of Supercharged polypeptides based Biomaterials: from Adhesives to Fibers. University of Groningen. https://doi.org/10.33612/diss.116872472
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Fabrication and Mechanical
properties of Supercharged
polypeptides based Biomaterials:
from Adhesives to Fibers
Paranimphs:
Hongyan Li
Yifei Fan
The work described in this thesis was carried out at the Zernike Institute
for Advanced Materials, University of Groningen, The Netherlands. This
work was financially supported by China Scholarship Council (CSC) with
the Grant Number 201507720025.
Cover designed by Jing Sun and Jiaying Han
Printing: Proefschriftmaken
Zernike Institute PhD thesis series 2020-06
ISSN:
1570-1530
ISBN: 978-94-034-2434-7 (electronic version)
ISBN: 978-94-034-2435-4 (printed version)
© Jing Sun, 2020
All rights reserved. No parts of this thesis may be reproduced of
transmitted in any form or any means, electronic or mechanical, without
permission of the author.
Fabrication and Mechanical
properties of Supercharged
polypeptides based Biomaterials:
from Adhesives to Fibers
PhD thesis
to obtain the degree of PhD at the
University of Groningen
on the authority of the
Rector Magnificus Prof. C. Wijmenga
and in accordance with
the decision by the College of Deans.
This thesis will be defended in public on
Monday 10 February 2020 at 9.00 hours
by
Jing Sun
born on 12 December 1989
In Anhui, China
Supervisor
Prof. A. Herrmann
Co-promoter
Dr. G. Portale
Assessment Committee
Prof. M.M.G. Kamperman
Prof. J. C. M. van Hest
Prof. K. Peneva
Dedicated to my beloved wife
and families
Contents
Chapter 1 ... 1 Introduction ... 1 Abstract ... 2 1.1 Introduction ... 3 1.2. Protein-based adhesives ... 41.2.1 Genetically engineered protein-based adhesives ... 4
1.2.2 Biomimetic adhesives ... 8
1.3. Protein-based fibers ... 12
1.3.1 Strategies for the fabrication of protein-based fibers ... 13
1.3.2 Regenerated protein fibers ... 14
1.3.3 Recombinant protein fibers ... 15
1.4 Conclusions ... 18
1.5 Motivation and thesis outline ... 20
References ... 22
Chapter 2 ... 27
Ultra-Strong Bio-Glue from Genetically Engineered Polypeptides ... 27
Abstract ... 28
2.1 Introduction ... 29
2.2 Results and Discussion ... 29
2.3 Conclusion ... 33
2.4 Experimental section ... 33
References ... 48
Chapter 3 ... 51
Biomedical Applications of Ultra-Strong Bio-Glue from Genetically Engineered Polypeptides . 51 Abstract ... 52
3.1 Introduction ... 53
3.2 Results and Discussion ... 53
3.3 Conclusion ... 57
3.4 Experimental section ... 57
References ... 66
Chapter 4 ... 69
Design and Synthesis of Biological Adhesives Based on Polypeptide-Surfactant Complexes ... 69
Abstract ... 70
4.1 Introduction ... 71
4.2 Results and discussions ... 72
4.3 Conclusion ... 78
4.4 Experimental section ... 79
Reference ... 103
Chapter 5 ... 105
Reversibly Photo-Modulating the Mechanics of Bioengineered Protein Fibers ... 105
Abstract ... 106
5.1 Introduction ... 107
5.2 Results and Discussions ... 108
5.3 Conclusion ... 113 5.4 Experimental section ... 113 References ... 129 Summary ... 131 Samenvatting ... 135 Acknowledgements ... 139
