University of Groningen
Supercharged proteins and polypeptides for advanced materials in chemistry and biology
Ma, Chao
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Publication date: 2019
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Ma, C. (2019). Supercharged proteins and polypeptides for advanced materials in chemistry and biology. University of Groningen.
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Supercharged Proteins and Polypeptides for
Advanced Materials in Chemistry and Biology
The work described in this thesis was performed in the Polymer Chemistry and
Bioengineering group of the Zernike Institute for Advanced Materials at the University
of Groningen, the Netherlands.
This work was supported financially by the Chinese Scholarship Council.
Cover design by: Guolong (Sedah) Mo and Chuyao Geng
Printed by IPSKAMP printing
Zernike Institute PhD thesis series 2019-19
ISSN 1570-1530
ISBN: 978-94-034-1789-9 (printed version)
ISBN: 978-94-034-1788-2 (electronic version)
Supercharged Proteins and
Polypeptides for Advanced
Materials in Chemistry and Biology
Phd thesis
to obtain the degree of PhD at the
University of Groningen
on the authority of the
Rector Magnificus prof. E. Sterken
and in accordance with
the decision by the College of Deans.
This thesis will be defended in public on
Friday 28 June 2019 at 11.00 hours
by
Chao Ma
born on 5 December 1988
in Shanxi, China
Supervisor
Prof. A. Herrmann
Co-supervisor
Prof. P. van Rijn
Assessment Committee
Prof. D. J. Slotboom
Prof. H. Boerner
Prof. U. Schwaneberg
Table of Contents
CHAPTER 1. INTRODUCTION
1.Introduction Of Supercharged Proteins ... 3
2.Supercharged Proteins In Nature ... 4
3.Engineering Supercharged Folded Proteins ... 13
4.Supercharging By Conjugation to A Natural Disordered Polypeptide ... 20
5.Engineering Supercharged Unstructured Polypeptides ... 23
6.Conclusions and Outlook... 36
7.Motivation and Thesis Outline ... 38
8.Reference ... 40
CHAPTER 2. TUNING ICE NUCLEATION WITH SUPERCHARGED POLYPEPTIDES 1.Introduction ... 51
2.Results and Discussion ... 52
3.Conclusion ... 60
4.Experimental Part and Supplementary Data ... 61
5.References ... 79
CHAPTER 3. A HYPOTHESIS-FREE SENSOR ARRAY DISCRIMINATES WHISKIES FOR BRAND, AGE, AND TASTE 1.Introduction ... 83
2.Results and Discussion ... 84
3.Conclusion ... 91
4.Experimental Part and Supplementary Data ... 91
CHAPTER 4. SELF-ASSEMBLY OF ELECTROSTATIC COCRYSTALS FROM SUPERCHARGED FUSION PEPTIDES AND PROTEIN CAGES
1.Introduction...115
2.Resultsand Discussion...116
3.Conclusion...122
4.Experimental Partand Supplementary Data...122
5.References...134
CHAPTER5.DE NOVO DESIGN OF A FREE-STANDING, SUPERCHARGED POLYPEPTIDE, PROTON CONDUCTING MEMBRANE 1.Introduction...141
2.Resultsand Discussion...142
3.Conclusion...150 4.Supplementary Data...151 5.References...167 SUMMARY...171 SAMENVATTING...175 PUBLICATION LIST...178 ACKNOWLEDGEMENTS...180
