University of Groningen
The role of microbe-matrix interactions in dairy starter culture functionality
Tarazanova, Mariya
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Publication date: 2018
Link to publication in University of Groningen/UMCG research database
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Tarazanova, M. (2018). The role of microbe-matrix interactions in dairy starter culture functionality. University of Groningen.
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The role of Microbe-Matrix interactions
in dairy starter culture functionality
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The research described in this thesis was conducted at NIZO BV (Ede, The Netherlands) and was embedded within the Molecular Genetics Group of the Faculty Mathematics and Natural Sciences, University of Groningen (Groningen, The Netherlands).
The research presented in this thesis was performed within the framework of TI Food and Nutrition (TiFN, Wageningen, The Netherlands), a public-private partnership on pre-competitive research in food and nutrition.
Industrial partners:
Printing of this thesis was financially supported by the Graduate School of Science (University of Groningen) and TiFN.
Cover Design: Milo van der Schuur, Ben Jansen, Mariya Tarazanova
The cover shows the 3D milk matrix fermented with Lactococcus lactis B40. The image was taken by Jan Klok/M.Tarazanova with confocal laser scanning microscopy (zoom 4, 13 μm deep into the sample) by merging 3 pictures of z-scan. The Argon laser was used to visualize the Syto 9-stained bacteria, while the DPSS 561 laser was used to visualize the milk protein and fat droplet in the matrix that were stained by the Acridine Orange and Rhodamine B, respectively. The objective lens used was an HCX PL APO 63×/1.2 /water CORR CS. Bacterial cells are green, proteins and lipids appear orange/red; the black areas represent the serum fraction. For other examples of fermented milk microstructure please see Chapter 4.
Layout: Ben Jansen & Mariya Tarazanova
Printed by: Ipskamp Drukkers B.V., Enschede, The Netherlands
ISBN (print): 978-94-034-0870-5
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The role of Microbe-Matrix
interactions in dairy starter culture
functionality
PhD thesis
to obtain the degree of PhD at the
University of Groningen
on the authority of the
Rector Magnificus Prof. E. Sterken
and in accordance with
the decision by the College of Deans.
This thesis will be defended in public on
Friday 12 October 2018 at 12.45 hours
by
Mariya Tarazanova
born on 25 February 1988
in Moscow, Russia
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Supervisor Prof. J. Kok Co-supervisor Dr. H. Bachmann Assessment Committee Prof. L. Dijkhuizen Prof. E.J. Smid Prof. E. Tsakalido
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Contents
CHAPTER 1 ... 1
GENERAL INTRODUCTION... 1
CHAPTER 2 ... 53
PLASMID COMPLEMENT OF LACTOCOCCUS LACTISNCDO712 REVEALS A NOVEL PILUS GENE CLUSTER ... 53
CHAPTER 3 ... 95
CELL SURFACE PROPERTIES OF LACTOCOCCUS LACTIS REVEAL MILK PROTEIN BINDING SPECIFICALLY EVOLVED IN DAIRY ISOLATES ... 95
CHAPTER 4 ... 139
ALTERING TEXTURAL PROPERTIES OF FERMENTED MILK BY USING SURFACE -ENGINEERED LACTOCOCCUS LACTIS ... 139
CHAPTER 5 ... 165
INFLUENCE OF LACTOCOCCAL SURFACE PROPERTIES ON CELL RETENTION AND DISTRIBUTION IN CHEESE CURD ... 165
CHAPTER 6 ... 183
BACTERIAL EMULSIFICATION BY AND TRANSCRIPTIONAL RESPONSE OF LACTOCOCCUS LACTIS RESIDING AT AN OIL-WATER INTERPHASE ... 183
CHAPTER 7 ... 209 GENERAL DISCUSSION ... 209 ADDENDA ... 221 Samenvatting ... 222 Summary ... 225 Резюме ... 228 Acknowledgements ... 232
About the author ... 234
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