Citation
Hassink, G. C. (2006, May 22). The role of the ubiquitin system in human
cytomegalovirus-mediated degradation of MHC class I heavy chains. Retrieved from
https://hdl.handle.net/1887/4414
Version:
Corrected Publisher’s Version
License:
Licence agreement concerning inclusion of doctoral thesis in the
Institutional Repository of the University of Leiden
Downloaded from:
https://hdl.handle.net/1887/4414
THE ROLE OF THE UBIQUITIN SYSTEM IN HUMAN CYTOMEGALOVIRUS-MEDIATED DEGRADATION OF
THE ROLE OF THE UBIQUITIN SYSTEM IN HUMAN CYTOMEGALOVIRUS-MEDIATED DEGRADATION OF
MHC CLASS I HEAVY CHAINS
PROEFSCHRIFT
ter verkrijging van
de graad van Doctor aan de UniversiteitLeiden, op gezag van de Rector Magnificus Dr.D.D.Breimer,
hoogleraar in de faculteitder W iskunde en Natuurwetenschappen en die der Geneeskunde,
volgens besluitvan hetCollege voor Promoties te verdedigen op maandag 22 mei2006
te klokke 14.15 uur
door
Gerrit Cornelis Hassink
Contents
Abbreviations 8
Chapter 1 Introduction.Partly published in Curr. Top. Microbiol.
Immunol. 1995, 300:57-94
9
Chapter 2 Rat Cytomegalovirus Induces a Temporal
Down-regulation of Major Histocompatibility Complex Class I CellSurface Expression.Vir. Immunol. 2005, 18:607-15
3 3
Chapter 3 Ubiquitination is essentialfor human cytomegalovirus
US11-mediated dislocation of MHC class I molecules from the endoplasmic reticulum to the cytosol.Biochem J. 2001, 358:369-77
4 9
Chapter 4 Human Hrd1 is an E3 ubiquitin ligase involved in
degradation of proteins from the Endoplasmic Reticulum.J.Biol.Chem. 2004, 279:3525-34
6 9
Chapter 5 TEB4 is a C4HC3 RING finger-containing ubiquitin
ligase of the endoplasmic reticulum.Biochem J. 2005, 388:647-55
95
Chapter 6 Ubiquitination of lysine residues within MHC class I
heavy chains is essentialfor dislocation by HCMV US2 but not by HCMV US11. Submitted
1 1 5
Chapter 7 Human cytomegalovirus-encoded US2 and US11 target
unassembled MHC class I heavy chains for degradation.Mol Immunol. 2006, 43:1258-66.
1 4 1
Chapter 8 MHC class I heavy chains are dislocated from the
endoplasmic reticulum to the cytosolin a vectorial fashion,commencing at the C-terminus.Submitted
1 5 9
Chapter 9 Summary and Discussion 1 81
Nederlandse samenvatting 2 0 1
Curriculum Vitae 2 1 1
8 HC HCMV HLA IFN IL IRES kDa mAb MHC MOI NK cell NP40 PDI PE ProtK TAP TM TNF Tx-100 US wt ZL3H ZLVS heavy chain
human cytomegalovirus human leucocyte antigen interferon
interleukin
internalribosomalentry site kilodalton
monoclonalantibody
major histocompatibility complex multiplicity of infection.
naturalkiller cell nonidetP-40
protein disulfide isomerase phycoerythrin
proteinase K
transporter associated with antigen processing transmembrane
tumor necrosis factor triton X-100
unique short wild type
carboxybenzyl-leucyl-leucyl-leucinal
