Perona Martinez, Felipe
DOI:
10.33612/diss.111974782
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Publication date: 2020
Link to publication in University of Groningen/UMCG research database
Citation for published version (APA):
Perona Martinez, F. (2020). Diamond magnetometry for sensing in biological environment. Rijksuniversiteit Groningen. https://doi.org/10.33612/diss.111974782
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Diamond Magnetometry
for sensing in biological
environment
retrieval system or transmitted in any form or by any means, without permission of the author and, when appropriate, the publisher holding the copyrights of the published articles.
Cover : Felipe Perona Mart´ınez ISBN : 978-94-034-2258-9 ISBN digital : 978-94-034-2257-2 This book was made in LATEX
Diamond Magnetometry
for sensing in biological
environment
Proefschrift
ter verkrijging van de graad van doctor aan de Rijksuniversiteit Groningen
op gezag van de
rector magnificus prof. dr. C. Wijmenga en volgens besluit van het College voor Promoties.
De openbare verdediging zal plaatsvinden op woensdag 22 januari 2020 om 14:30 uur
door
Felipe Patricio Perona Mart´ınez
geboren op 29 september 1979Prof. dr. Y. Ren Prof. dr. P. Rudolf Prof. dr. K. Haenen
Paranimfen Kiran van der Laan Alina Sigaeva
Contents
1 Introduction 1
2 Improving the cellular uptake 5
2.1 Introduction . . . 7
2.2 Results and discussion . . . 9
2.2.1 Characterization of coated nanodiamonds with AFM 9 2.2.2 Colloidal stability of protein-based polymer coated nanodiamonds . . . 10
2.2.3 Binding strength of protein polymers to nanodiamonds 12 2.2.4 Photoluminescence properties of protein polymer coa-ted nanodiamonds . . . 13
2.2.5 Cytotoxicity of protein polymer coated nanodiamonds 14 2.2.6 Cell uptake of bare and protein polymer coated nan-odiamonds . . . 15 2.3 Experimental Details . . . 18 2.3.1 Materials . . . 18 2.3.2 Methods . . . 19 2.4 Concluding remarks . . . 21 2.5 Supporting Information . . . 23
2.5.1 Binding of the C4 protein polymer . . . 23
2.5.2 Clusters of nanodiamonds . . . 23
2.5.3 Magneto-optical properties . . . 24
2.5.4 Protein polymer binding stability at different pH . . 25
3 Sample Preparation in Proteomics 33 3.1 Introduction . . . 35
3.2 Materials and Methods . . . 36
3.2.1 Materials . . . 36
3.2.2 Sample preparation . . . 37
3.2.3 Sample preparation with carbon black . . . 37
3.2.4 Protein analysis . . . 37 vii
relaxation constant T1 . . . 58
4.2.2 Performance of the NVs in biologically relevant con-ditions . . . 59
4.2.3 Measuring free radicals in situ . . . 62
4.3 Experimental Details . . . 65
4.3.1 Nanodiamonds . . . 65
4.3.2 T1 measurements . . . 65
4.3.3 GdCl3 sensitivity in different conditions . . . 65
4.3.4 Hydrogen Peroxide and UV light . . . 66
4.3.5 Measuring the hydroxyl radical by HPF . . . 66
4.3.6 Measuring the concentration of hydroxyl radical by HTA . . . 66
4.3.7 Measuring the Fenton reaction by diamond magne-tometry . . . 67
4.4 Concluding remarks . . . 67
4.5 Supporting Information . . . 68
4.5.1 Measuring free radicals in-situ . . . 68
4.5.2 Measuring the concentration of hydroxyl radical by HTA . . . 68
4.5.3 Size distribution of the nanodiamonds . . . 70
5 Discussion 75 6 Summary — Samenvatting 81 6.1 Summary . . . 81
6.2 Samenvatting . . . 84
Acknowledgments 89
About the author 93
