University of Groningen
Development of genetic manipulation tools in Macrostomum lignano for dissection of
molecular mechanisms of regeneration
Wudarski, Jakub
IMPORTANT NOTE: You are advised to consult the publisher's version (publisher's PDF) if you wish to cite from it. Please check the document version below.
Document Version
Publisher's PDF, also known as Version of record
Publication date: 2019
Link to publication in University of Groningen/UMCG research database
Citation for published version (APA):
Wudarski, J. (2019). Development of genetic manipulation tools in Macrostomum lignano for dissection of molecular mechanisms of regeneration. Rijksuniversiteit Groningen.
Copyright
Other than for strictly personal use, it is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), unless the work is under an open content license (like Creative Commons).
Take-down policy
If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim.
Downloaded from the University of Groningen/UMCG research database (Pure): http://www.rug.nl/research/portal. For technical reasons the number of authors shown on this cover page is limited to 10 maximum.
1) Recent progress in the development of molecular tools and resources for Macrostomum lignano makes it a convenient model to study regeneration and stem cell biology. (This thesis)
2) Microinjection technique can be used to deliver biologically relevant materials into single-cell stage M.lignano embryos. (This thesis)
3) Altering the temperature conditions can be used to shorten the time required to establish M. lignano cultures, store important lines and optimize the microinjection procedure. (This thesis)
4) Macrostomum lignano lines expressing fluorescent protein in a tissue-specific manner can be used to efficiently sort different cell types and analyze their transcriptomes. (This thesis)
5) Hsp20 promoter can be used as an inducible promoter to drive the expression of desired gene. (This thesis) 6) Pursue what is meaningful (not what is expedient). (Jordan Peterson)
