University of Groningen
Development of genetic manipulation tools in Macrostomum lignano for dissection of
molecular mechanisms of regeneration
Wudarski, Jakub
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Publication date: 2019
Link to publication in University of Groningen/UMCG research database
Citation for published version (APA):
Wudarski, J. (2019). Development of genetic manipulation tools in Macrostomum lignano for dissection of molecular mechanisms of regeneration. Rijksuniversiteit Groningen.
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Development of genetic
manipulation tools in
Macrostomum lignano for dissection
of molecular mechanisms of
regeneration
ISBN (printed version): 978-94-034-1318-1
ISBN (electronic version): 978-94-034-1317-4
Development of genetic manipulation
tools in Macrostomum lignano for
dissection of molecular mechanisms of
regeneration
PhD thesis
to obtain the degree of PhD at the University of Groningen
on the authority of the Rector Magnificus Prof. E. Sterken
and in accordance with the decision by the College of Deans. This thesis will be defended in public on Monday 14 January 2019 at 12.45 hours
by
Jakub Wudarski
born on 8 January 1986Supervisor
Prof. E. Berezikov
Co-supervisor
Prof. V. Guryev
Assessment Committee
Prof. E.A.A. Nollen Prof. F.M. Reggiori Prof. M. Tijsterman
To my family
Mojej Rodzince
CONTENTS
CHAPTER
1
9
General introduction and the outline of the thesis
CHAPTER
2
15
The regenerative flatworm Macrostomum lignano, a model organism with
high experimental potential
CHAPTER
3
33
Efficient transgenesis and annotated genome sequence of the regenerative flatworm model Macrostomum lignano
CHAPTER
4
71
Proof of principle for transposon-mediated transgenesis in
Macrostomum lignano
CHAPTER
5
85
Influence of temperature on the development, reproduction and regeneration in the flatworm model organism Macrostomum lignano
CHAPTER
6
103
Flourescentce activated cell sorting is an efficient approach for establishing transcriptional profiles of various tissues in the flatworm
Macrostomum lignano
CHAPTER
7
127
Summary and Discussion Future perspectives
