University of Groningen
Functional role of lipids in bacterial protein translocation
Koch, Sabrina
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2019
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Koch, S. (2019). Functional role of lipids in bacterial protein translocation. University of Groningen.
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PROPOSITIONS
Associated with the PhD thesis
Functional role of lipids in bacterial protein translocation
Sabrina Koch
1) Detergents can alter functional and structural properties of
mem-brane proteins. Therefore, there is a great demand for model lipid bilayers to study membrane proteins in a physiologically relevant environment. – Chapter 1
2) SecA binding and translocation are dependent on the
phospho-lipid charge, but do not rely on a specific anionic phosphophospho-lipid type. – Chapter 3
3) SecA binds via its positively charged N-terminus to anionic lipids
in the cytoplasmic membrane. This binding event primes SecA for high affinity binding to SecYEG – Chapter 2
4) Anionic lipids that bind in the vicinity of the lateral gate of SecY
may stabilize the pre-open state of the lateral gate of the SecY channel and promote correct positioning of an incoming signal sequence of a preprotein. – Chapter 3
5) Single SecYEG complexes diffuse in interchangeable slow and
fast mobility modes. – Chapter 4
6) The quality of research should not have to suffer from unrealistic
demands and high pressure of the academic system.
7) One should not pursue a PhD to gain social status or financial
security. There are easier and faster ways to become rich and famous.
8) Intense competition and “publish or perish” practices spoil the
freedom, motivation and curiosity of young scientists that need to seek, explore and understand nature’s hidden treasures.
9) I often think the greatest failure in life could very well be, never
having failed. It means you never dared to dream big, never tried something extraordinary. Learn to embrace failure and be proud of failing, because there is such a thing as failing upwards. Just don’t let the failures debilitate you. Learn from them, let them motivate you- Embrace failure and in some ways you will never fail – Jimmy Chin
