University of Groningen
Antimicrobial and nanoparticle penetration and killing in infectious biofilms
Rozenbaum, René Theodoor
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Publication date: 2019
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Rozenbaum, R. T. (2019). Antimicrobial and nanoparticle penetration and killing in infectious biofilms. Rijksuniversiteit Groningen.
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Antimicrobial and nanoparticle penetration
and killing in infectious biofilms
This research has received funding from the European Union’s Seventh Framework Program (FP7/2007-2013) under grant agreement no 604182.
It was carried out within the project FORMAMP - Innovative Nanoformulation of Antimicrobial Peptides to Treat Bacterial Infectious Diseases.
Antimicrobial and nanoparticle penetration and killing in infectious biofilms
University Medical Center Groningen, University of Groningen Groningen, The Netherlands
Copyright © 2019 by René Theodoor Rozenbaum
Cover: Live/Dead stained Pseudomonas aeruginosa adhered on a glass plate Printed by Gilderprint
ISBN (printed version): 978-94-6323-635-5 ISBN (electronic version): 978-94-6323-639-3
Antimicrobial and nanoparticle penetration
and killing in infectious biofilms
Proefschrift
ter verkrijging van de graad van doctor aan de Rijksuniversiteit Groningen
op gezag van de
rector magnificus prof. dr. E. Sterken en volgens besluit van het College voor Promoties.
De openbare verdediging zal plaatsvinden op woensdag 5 juni 2019 om 12:45 uur
door
René Theodoor Rozenbaum
geboren op 22 augustus 1989 te Middelburg
This research has received funding from the European Union’s Seventh Framework Program (FP7/2007-2013) under grant agreement no 604182.
It was carried out within the project FORMAMP - Innovative Nanoformulation of Antimicrobial Peptides to Treat Bacterial Infectious Diseases.
Antimicrobial and nanoparticle penetration and killing in infectious biofilms
University Medical Center Groningen, University of Groningen Groningen, The Netherlands
Copyright © 2019 by René Theodoor Rozenbaum
Cover: Live/Dead stained Pseudomonas aeruginosa adhered on a glass plate Printed by Gilderprint
ISBN (printed version): 978-94-6323-635-5 ISBN (electronic version): 978-94-6323-639-3
Antimicrobial and nanoparticle penetration
and killing in infectious biofilms
Proefschrift
ter verkrijging van de graad van doctor aan de Rijksuniversiteit Groningen
op gezag van de
rector magnificus prof. dr. E. Sterken en volgens besluit van het College voor Promoties.
De openbare verdediging zal plaatsvinden op woensdag 5 juni 2019 om 12:45 uur
door
René Theodoor Rozenbaum
geboren op 22 augustus 1989 te Middelburg
Promotores
Prof. dr. H.C. van der Mei Prof. dr. ir. H.J. Busscher
Copromotor
Dr. P.K. Sharma
Beoordelingscommissie
Prof. dr. J.M. van Dijl Prof. dr. M. Malmsten Prof. dr. M.M.G. Kamperman
Paranimfen
Joyce Zwerver Maaike Rozenbaum
Promotores
Prof. dr. H.C. van der Mei Prof. dr. ir. H.J. Busscher
Copromotor
Dr. P.K. Sharma
Beoordelingscommissie
Prof. dr. J.M. van Dijl Prof. dr. M. Malmsten Prof. dr. M.M.G. Kamperman
Paranimfen
Joyce Zwerver Maaike Rozenbaum
Table of Contents
Chapter 1 General introduction 9
Chapter 2 A constant depth film fermenter to grow microbial biofilms
R.T. Rozenbaum, W. Woudstra, E.D. de Jong, H.C. van der Mei, H.J. Busscher and P.K. Sharma
Nature protocol exchange, 2017, 10.1038/protex.2017.024
15
Chapter 3 Bacterial density and biofilm structure determined by optical coherence tomography
J. Hou, C. Wang, R.T. Rozenbaum, N. Gusnaniar, E.D. de Jong, W. Woudstra, G. Geertsema-Doornbusch, J. Atema-Smit, J. Sjollema, Y. Ren, H.J. Busscher and H.C.van der Mei
Submitted
37
Chapter 4 Role of viscoelasticity in bacterial killing by antimicrobials in
differently grown Pseudomonas aeruginosa biofilms
R.T. Rozenbaum, H.C. van der Mei, W. Woudstra, E.D. de Jong, H.J. Busscher and P.K. Sharma
Antimicrob. Agents Chemother., 2019, 63 (4)
61
Chapter 5 Penetration and accumulation of dendrons with different peripheral
composition in Pseudomonas aeruginosa biofilms
R.T. Rozenbaum, O.C.J. Andrén, H.C. van der Mei, W. Woudstra, H.J. Busscher, M. Malkoch and P.K. Sharma
In revision
81
Chapter 6 Antimicrobial synergy of monolaurin lipid nanocapsules with
adsorbed antimicrobial peptides against Staphylococcus aureus biofilms in vitro and in vivo
R.T. Rozenbaum, L. Su, A. Umerska, M.Eveillard, J. Håkansson, M. Mahlapuu, F. Huang, J. Liu, Z. Zhang, L. Shi, H.C. van der Mei. H.J. Busscher and P.K. Sharma
J. Control. Release, 2018, 293; 73-83
99
Chapter 7 General discussion Summary Samenvatting Acknowledgements 127 135 139 145
Table of Contents
Chapter 1 General introduction 9
Chapter 2 A constant depth film fermenter to grow microbial biofilms
R.T. Rozenbaum, W. Woudstra, E.D. de Jong, H.C. van der Mei, H.J. Busscher and P.K. Sharma
Nature protocol exchange, 2017, 10.1038/protex.2017.024
15
Chapter 3 Bacterial density and biofilm structure determined by optical coherence tomography
J. Hou, C. Wang, R.T. Rozenbaum, N. Gusnaniar, E.D. de Jong, W. Woudstra, G. Geertsema-Doornbusch, J. Atema-Smit, J. Sjollema, Y. Ren, H.J. Busscher and H.C.van der Mei
Submitted
37
Chapter 4 Role of viscoelasticity in bacterial killing by antimicrobials in
differently grown Pseudomonas aeruginosa biofilms
R.T. Rozenbaum, H.C. van der Mei, W. Woudstra, E.D. de Jong, H.J. Busscher and P.K. Sharma
Antimicrob. Agents Chemother., 2019, 63 (4)
61
Chapter 5 Penetration and accumulation of dendrons with different peripheral
composition in Pseudomonas aeruginosa biofilms
R.T. Rozenbaum, O.C.J. Andrén, H.C. van der Mei, W. Woudstra, H.J. Busscher, M. Malkoch and P.K. Sharma
In revision
81
Chapter 6 Antimicrobial synergy of monolaurin lipid nanocapsules with
adsorbed antimicrobial peptides against Staphylococcus aureus biofilms in vitro and in vivo
R.T. Rozenbaum, L. Su, A. Umerska, M.Eveillard, J. Håkansson, M. Mahlapuu, F. Huang, J. Liu, Z. Zhang, L. Shi, H.C. van der Mei. H.J. Busscher and P.K. Sharma
J. Control. Release, 2018, 293; 73-83
99
Chapter 7 General discussion Summary Samenvatting Acknowledgements 127 135 139 145
