Cover Page
The handle http://hdl.handle.net/1887/136520 holds various files of this Leiden University
dissertation.
Author: Hafkenscheid, L.
Title: Anti Citrullinated Protein Antibodies-IgG variable domain glycosylation in
rheumatoid arthritis
CURRICULUM VITAE
LIST OF PUBLICATIONS
1. On the presence of HLA-SE alleles and ACPA-IgG variable domain glycosylation in the phase preceding the development of rheumatoid arthritis. Kissel T, van Schie K.A, Hafkenscheid L, Lundquist A, Kokkonen H, Wuhrer M, Huizinga T.W, Scherer H.U, Toes R, Rantapää-Dahlqvist S. Ann Rheum Dis. 2019 Aug 30. pii: annrheumdis-2019-215698. doi: 10.1136/annrheumdis-2019-215698. PMID: 31471298. 2. Different classes of anti-modified protein antibodies are induced on exposure to
antigens expressing only one type of modification. Kampstra A.S.B, Dekkers J.S, Volkov M, Dorjée A.L, Hafkenscheid L, Kempers A.C, van Delft M, Kissel T, Reijm S, Janssen G.M.C, van Veelen P.A, Bang H, Huizinga T.W.J, Trouw L.A, van der Woude D, Toes R.E.M. Ann Rheum Dis. 2019 Jul;78(7):908-916. doi: 10.1136/ annrheumdis-2018-214950. PMID: 31151934.
3. N-Linked Glycans in the Variable Domain of IgG Anti-Citrullinated Protein Antibodies Predict the Development of Rheumatoid Arthritis. Hafkenscheid L, de Moel E, Smolik I, Tanner S, Meng X, Jansen B.C, Bondt A, Wuhrer M, Huizinga T.W.J, Toes R.E.M, El-Gabalawy H, Scherer H.U. Arthritis Rheumatol. 2019 May 8. doi: 10.1002/ art.40920. PMID: 31067000
4. HappyTools: A software for high-throughput HPLC data processing and quantitation. Jansen B.C, Hafkenscheid L, Bondt A, Gardner R.A, Hendel J.L, Wuhrer M, Spencer D.I.R. PLoS One. 2018 Jul 6;13(7):e0200280. doi: 10.1371/journal.pone.0200280. eCollection 2018. PMID: 29979768
5. ACPA IgG galactosylation associates with disease activity in pregnant patients with rheumatoid arthritis. Bondt A, Hafkenscheid L, Falck D, Kuijper T.M, Rombouts Y, Hazes .J.M.W, Wuhrer M, Dolhain R.J.E.M. Ann Rheum Dis. 2018 Aug;77(8):1130-1136. doi: 10.1136/annrheumdis-2018-212946. PMID: 29615411.
Appendices
8. Variable domain glycosylation of ACPA-IgG: A missing link in the maturation of the ACPA response? Kempers AC, Hafkenscheid L, Scherer HU, Toes REM. Clin Immunol. 2018 Jan;186:34-37. doi: 10.1016/j.clim.2017.09.001. Review. PMID: 28882619. 9. B-cell receptor sequencing of anti-citrullinated protein antibody (ACPA) IgG-expressing
B cells indicates a selective advantage for the introduction of N-glycosylation sites during somatic hypermutation. Vergroesen R.D*, Slot L.M*, Hafkenscheid L*, Koning M.T, van der Voort E.I.H, Grooff C.A, Zervakis G, Veelken H, Huizinga T.W.J, Rispens T, Scherer H.U, Toes R.E.M. Ann Rheum Dis. 2018 Jun;77(6):956-958. doi: 10.1136/ annrheumdis-2017-212052. PMID:28835463 *shared first
10. The extensive glycosylation of the ACPA variable domain observed for ACPA-IgG is absent from ACPA-IgM. Kempers AC, Hafkenscheid L, Dorjée A.L, Moutousidou E, van de Bovenkamp F.S, Rispens T, Trouw L.A, van Oosterhout M, Huizinga T.W, Toes R, Scherer H.U. Ann Rheum Dis. 2018 Jul;77(7):1087-1088. doi: 10.1136/ annrheumdis-2017-211533. Epub 2017 Jul 26. PMID: 28747327.
11. Structural Analysis of Variable Domain Glycosylation of Anti-Citrullinated Protein Antibodies in Rheumatoid Arthritis Reveals the Presence of Highly Sialylated Glycans. Hafkenscheid L, Bondt A, Scherer H.U, Huizinga T.W, Wuhrer M, Toes R.E, Rombouts Y. Mol Cell Proteomics. 2017 Feb;16(2):278-287. doi: 10.1074/mcp.M116.062919. Epub 2016 Dec 12. PMID: 27956708.
12. The Emerging Importance of IgG Fab Glycosylation in Immunity. van de Bovenkamp F.S*, Hafkenscheid L*, Rispens T, Rombouts Y. J Immunol. 2016 Feb 15;196(4):1435-41. doi: 10.4049/jimmunol.1502136. Review. PMID: 26851295 *shared first
ACKNOWLEDGEMENTS
I would like to start with thanking my promotor, Prof. René Toes. You gave me the opportunity to work on this project. You always knew how to guide me in the right direction and kept me focussed.
Secondly, I would like to thank my co-promotors: Dr. Uli Scherer and Dr. Yoann Rombouts. Yoann, you convinced me to do a PhD in the field of glycobiology, which I am grateful for. Together with Emeline, you showed me how interesting glycans are. Uli, I really liked the discussions we had about my research. You taught me that there are multiple angles to look at scientific research, including your clinical point of view.
I like to thank the rheumatology Lab, you were/are so kind, hard working, helpful and there was always time for a social event. Most of you I consider as friends and I’m happy to see that our paths are crossing during our careers. For my fellow PhD students that either are still working on their projects, have already obtained their title or working to get it: a PhD is definitely a process, but you all made it worthwhile and I hope you feel the same way. For the postdocs that I worked with, thank you for all the knowledge and being an example for me. To all the rheumatology technicians, thank you for all your help during my project and I never hesitated to ask for help, as you were always willing or offering to help me.
I also like to thank the CPM department. Prof Manfred Wuhrer, I like to thank you for the opportunity that I could work in your lab. To all the PhDs, Postdoc and technicians of the CPM department, thank you for letting me feel welcome in the lab as the stranger from the rheumatology department. In addition, I learned a lot about glycosylation that I still apply in my current work today.
