University of Groningen Novel fluorescent probes and analysis methods for single-molecule and single-cell microscopy Smit, Jochem

University of Groningen

Novel fluorescent probes and analysis methods for single-molecule and single-cell

microscopy

Smit, Jochem

DOI:

10.33612/diss.102269758

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Publication date: 2019

Link to publication in University of Groningen/UMCG research database

Citation for published version (APA):

Smit, J. (2019). Novel fluorescent probes and analysis methods for single-molecule and single-cell microscopy. Rijksuniversiteit Groningen. https://doi.org/10.33612/diss.102269758

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Stellingen

Novel fluorescent probes and analysis methods for

single-molecule and single-cell microscopy

Jochem H. Smit

1. The spatiotemporal resolution of optical microscopes is limited by the number of photons collected and therefore the photostability of the probes used. (Chapter 2)

2. In order to more effectively reach to practical applications, photostability studies need to move away from model systems in the absence of oxygen and towards more realistic in vivo situations. (Chapter 3)

3. The scientific process can be significantly sped up by standardization, documen-tation and distribution of data analysis software. (Chapter 4)

4. Fundamentally new antimicrobial mechanisms are needed to combat the rise of antimicrobial resistance; photodynamic therapy can be one of these mechanisms. (Chapter 5)

5. The focus on performance metrics of individual scientists is a disincentive for collaboration and altruism for the benefit of the scientific community.

6. Efficient programming is balancing time spent googling versus time spend writing code.

7. Only the first scientist is in the position to question everything - the rest have to choose when to be critical.

8. In the hands of a chemist, aluminium foil is a heat insulator, in the hands of a physicist aluminium foil is a heat conductor.

9. Allosteric effects are biology’s spooky action at a distance.

10. Great care needs to be taken when labelling – for example, the labelling of a pen in a lab will significantly reduce its apparent diffusion coefficient.