University of Groningen Exploring deazaflavoenzymes as biocatalysts Kumar, Hemant

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As he puts it: ‘The Kantian idea of moral autonomy does not primarily enlighten us about how we should actually structure our life and actions, but about the

The research described in this thesis has been carried out at Molecular Enzymology group of Groningen Biotechnology and Biomolecular Sciences Institute, University of the

Based on homology searches using the sequences of known deazaflavoproteins, F420 producing bacteria are predicted to con- tain many (uncharacterized) F420-dependent enzymes. Future

immobilized column. FMN and other flavin binding proteins may also bind, but with lower af- finity. b) unbound or loosely bound proteins will be removed during the washing step.

All the generated Tfu-FNO mutants had melting temperatures similar to the wild-type enzyme (data not shown).. This indicates that FNO is remarkably thermostable and is

Three specific ancestral states were selected for further analysis: the node between the dehydrogenases and reductases (named AncDR), the cenancestor of the three