• No results found

University of Groningen The roles of MYO5B in epithelial cells and the intestine Leng, Changsen

N/A
N/A
Protected

Academic year: 2021

Share "University of Groningen The roles of MYO5B in epithelial cells and the intestine Leng, Changsen"

Copied!
9
0
0

Bezig met laden.... (Bekijk nu de volledige tekst)

Hele tekst

(1)

University of Groningen

The roles of MYO5B in epithelial cells and the intestine

Leng, Changsen

DOI:

10.33612/diss.127906021

IMPORTANT NOTE: You are advised to consult the publisher's version (publisher's PDF) if you wish to cite from it. Please check the document version below.

Document Version

Publisher's PDF, also known as Version of record

Publication date: 2020

Link to publication in University of Groningen/UMCG research database

Citation for published version (APA):

Leng, C. (2020). The roles of MYO5B in epithelial cells and the intestine: A focus on microvillus inclusion disease. University of Groningen. https://doi.org/10.33612/diss.127906021

Copyright

Other than for strictly personal use, it is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), unless the work is under an open content license (like Creative Commons).

Take-down policy

If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim.

Downloaded from the University of Groningen/UMCG research database (Pure): http://www.rug.nl/research/portal. For technical reasons the number of authors shown on this cover page is limited to 10 maximum.

(2)

The roles of MYO5B in epithelial

cells and the intestine

A focus on microvillus inclusion disease

(3)

The roles of MYO5B in epithelial cells and the intestine

A focus on microvillus inclusion disease

This research was financially supported by: China Scholarship Council (CSC), Graduate School of Medical Science, University of Groningen, De Stichting De Cock-Hadders, TKO Strong Foundation, The Daniel Courtney Trust

The experiments described in this thesis were conducted at: Department of Biomedical Sciences of Cells and Systems, section Molecular Cell Biology, Groningen University Institute for Drug Exploration (GUIDE). University of Groningen, University Medical Center Groningen, Groningen, the Netherlands

ISBN: 978-94-034-2688-4 (Printed) ISBN: 978-94-034-2689-1 (Digital)

Thesis design: Changsen Leng

Cover: Fluorescent images of control mouse intestine (left) and myo5b knockout mouse intestine (right) with DAPI (blue) and NHE3 (green) staining.

Printing: Ridderprint B.V., Ridderkerk, the Netherlands

Copyright © 2020 Changsen Leng. All rights reserved. No part of this book may be reproduced or transmitted in any form or by any means without written permission of the author.

(4)

The roles of MYO5B in epithelial

cells and the intestine

A focus on microvillus inclusion disease

PhD thesis

to obtain the degree of PhD at the

University of Groningen

on the authority of the

Rector Magnificus Prof. C. Wijmenga

and in accordance with

the decision by the College of Deans.

This thesis will be defended in public on

Monday 29 June 2020 at 9.00 hours

by

Changsen Leng

born on 3 March 1990

in Henan, China

(5)

Supervisors

Prof. S.C.D. van IJzendoorn

Dr. B.N.G. Giepmans

Assessment Committee

Prof. K.N. Faber

Prof. R.H.J. Houwen

Prof. J. Klumperman

(6)

Paranimfen:

Yue Sun

Karin Klappe

(7)
(8)

Contents

Chapter 1

9

Introduction and scope of the thesis

Chapter 2

25

Pharmacological interventions in microvillus inclusion disease: where do we

stand and where to go

Chapter 3

55

Loss of MYO5B impairs lysosome integrity and enterocyte viability in

microvillus inclusion disease

Chapter 4

97

Antioxidant treatment as a novel therapeutic option for microvillus inclusion

disease

Chapter 5

121

Loss of MYO5B expression deregulates late endosome size which hinders

mitotic spindle orientation

Chapter 6

171

Loss function of MYO5B sensitizes colorectal cancer Caco2 cells to cisplatin

Chapter 7

191

Summary and perspectives

Nederlandse samenvatting 203

Acknowledgements 215

(9)

Referenties

GERELATEERDE DOCUMENTEN

The roles of MYO5B in epithelial cells and the intestine: A focus on microvillus inclusion disease.. University

We investigated the P-gp/Cyp3a interplay and related DDIs with different P- gp inhibitors at the various regions of rat intestine ex vivo using Precision-Cut Intestinal

In support of a causal role for hepatic steatosis in the development of insulin resistance, an improvement in hepatic steatosis and NAFLD was associated with increased

To study the differences in P-gp activity in the different intestinal regions, slices were prepared from duodenum, jejunum, ileum and colon and pre- incubated

In this review the application of the more recently developed model of precision- cut intestinal slices (PCIS) for drug transport, metabolism and toxico logy

Moreover, to evaluate the influence of R123 on the viability of the slices during incubation, ATP content was measured after 3 h of incubation with or without R123

In view of the primary role of macrophages in hepatic inflammation, we also transplanted bone marrow from Cmklr1 knock-out (Cmklr1 -/- ) mice and wild type (WT) mice

The basal lamina is composed of different molecules which include integrins; they serve not only as the connection between the ECM and the epithelial cells but also as initiator