University of Groningen
Mutational impact of classical strain improvement on Penicillium chrysogenum
Wu, Min
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Publication date:
2019
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Wu, M. (2019). Mutational impact of classical strain improvement on Penicillium chrysogenum. University of
Groningen.
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Propositions associated with the PhD thesis
Mutational impact of classical strain improvement on Penicillium chrysogenum
Min Wu
1. Integration of genomic, transcriptomic and proteomic data has revealed many beneficial changes during classical strain improvement of industrial P.
chrysogenum strains for enhanced penicillin production. – Chapter 1
2. Classical strain improvement resulted in the inactivation of metabolic pathways that compete with the cysteine biosynthesis pathways for precursors, thereby contributing to an enhanced availability of cysteine for penicillin production. – Chapter 2
3. Mutational inactivation of the direct sulfhydrylation pathway results in slower cell growth and reduced secondary metabolite production which can be restored by externally added cysteine. – Chapter 3
4. Cysteine in medium does not rescue the growth of the P. chrysogenum strain with a deletion of the Pc21g18210 gene that encodes a homoserine O-acetyltransferase likely due to the absence of a functional cystathionine γ-synthase that converts cysteine into cystathionine. – Chapter 3
5. Methionine in medium may rescue the growth of the P. chrysogenum strain with a deletion of the Pc21g18210 gene that encodes a homoserine O-acetyltransferase because interconversion of sulfur amino acids (methionine-homocysteine-cysteine) occurs in the filamentous fungi. – Chapter 3
6. Inactivation of the ribose-5-phosphate isomerase B enzyme (RpiB) during classical strain improvement has little effect on metabolism, suggesting that RpiA is the main isomerase of the pentose phosphate pathway. – Chapter 4
7. “牵一发而动全身”-“Pull one hair and the whole body is affected”.
