Analysis of gene expression in the outer cell layers of Arabidopsis roots during lateral root development

Analysis of gene expression in the outer cell layers of Arabidopsis roots during lateral root development..

Hoewel in theorie verhoogde celdelingsactiviteit in de pericykel een signaal zou kunnen genereren dat door cellen in de buitenste weefsellagen wordt waargenomen bleek de AIR1 en

Neuteboom LW, Veth-Tello LM, Clijdesdale OR, Hooykaas PJJ, van der Zaal BJ (1999b) A novel subtilisin-like protease gene from Arabidopsis thaliana is expressed at sites of

The identification of AIR1A, AIR1B and AIR3, three auxin-responsive genes from Arabidopsis which are expressed specifically in the outer cell layers of the parental root at sites

However, measurements of the lateral root density (visible lateral roots per cm primary root) showed that overexpression of AIR3 had no effect on the number of emerged laterals:

There is no evidence for the hypothesis that genes are activated in the outer cell layers of the root in response to the damage caused by the emerging lateral root as suggested

FASTA analysis indicates that AIR1A, AIR1B and AIR1A-like are the only genes encoding CLCT proteins lacking a proline- or glycine-rich region in the Arabidopsis genome.

The other mutant backgrounds, including slr-1 (Figure 2d), showed the same GUS expression as similarly treated wild type plants, thus a ‘ringed’ pattern in non-induced seedlings