UvA-DARE is a service provided by the library of the University of Amsterdam (https://dare.uva.nl)
UvA-DARE (Digital Academic Repository)
Studies on a bacterial photosensor
Kort, R.
Publication date
1999
Link to publication
Citation for published version (APA):
Kort, R. (1999). Studies on a bacterial photosensor.
General rights
It is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s)
and/or copyright holder(s), other than for strictly personal, individual use, unless the work is under an open
content license (like Creative Commons).
Disclaimer/Complaints regulations
If you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please
let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material
inaccessible and/or remove it from the website. Please Ask the Library: https://uba.uva.nl/en/contact, or a letter
to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You
will be contacted as soon as possible.
Studies on a bacterial photosensor
1 General introduction
1.1 Purple bacteria
Life in the microbial world 3 Anoxygenic photosynthetic prokaryotes 3
Molecular genetics in Rhodobacter sphaeroides 6 1.2 How bacteria respond to their ambient environment
Sensing and signaling in bacteria: two-component systems 9 Chemotaxis: the enteric paradigm versus Rhodobacter sphaeroides 14
Phototaxis in purple bacteria 19 1.3 Photoactive yellow protein: a bacterial photosensor
Discovery and diversity 21 Structure and photocycle 24
The function 27 1.4 Outline of this thesis 28
2 The xanthopsin protein family: a new member in Rhodobacter sphaeroides
2.1 The xanthopsins: a new family of eubacterial blue-light photosensors 39 2.2 Sequence, chromophore extraction and 3-D model of the photoactive
yellow protein from Rhodobacter sphaeroides 49
3 Light-induced motility and adaptation responses in Rhodobacter sphaeroides
3.1 Physiological and genetic characterization of blue-light responses 57
3.2 Light-induced modulation of the release of 3H-methanol 73
4 Structural events associated with the photocycle of a xanthopsin
4.1 Evidence for trans-cis isomerization of thep-coumaric acid chromophore as
the photochemical basis of the photocycle of photoactive yellow protein 83 4.2 Trans/cis (Z/E) photoisomerization of the chromophore of photoactive
yellow protein is not a prerequisite for the initiation of the photocycle
of this photoreceptor protein 89 4.3 Time-resolved X-ray crystallography at the European Synchrotron Radiation
Facility: a discussion on structural events in the photocycle of a xanthopsin 95
Acknowledgements 105
Summary 107 Samenvatting (Dutch summary) 108
List of publications and abstracts 110
