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The production and purification of functional steroid hormone receptor ligand binding domains towards the development of a biological endocrine disruptor detection system

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The Production and Purification of Functional

Steroid Hormone Receptor Ligand Binding

Domains towards the Development of a Biological

Endocrine Disruptor Detection System

by

Timo Tait

Thesis presented in fulfilment of the requirements for the degree of Master of Science in the Faculty of Science at Stellenbosch University

Supervisor: Prof Pieter Swart

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496. Tait, T., Truebody, B., and Swart, P. (2014) Development of an immobilized receptor based EDC detection kit - Manufacture of PVP/PSMA contactor surfaces and the production of recombinant nuclear receptor ligand binding domains. Rep to WRC, 1–39

497. Kong, E. H., Heldring, N., Gustafsson, J.-A., Treuter, E., Hubbard, R. E., and Pike, A. C. W. (2005) Delineation of a unique protein-protein interaction site on the surface of the estrogen receptor. Proc. Natl. Acad. Sci. U. S. A. 102, 3593–3598

498. Norris, J. D., Paige, L. A., Christensen, D. J., Chang, C., Huacani, M. R., Fan, D., Hamilton, P. T., Fowlkes, D. M., and Mcdonnell, D. P. (1999) Peptide Antagonists of the Human Estrogen

Receptor. Science (80-. ). 285, 744–746

499. Huang, H.-J., Norris, J. D., and McDonnell, D. P. (2002) Identification of a negative regulatory surface within estrogen receptor alpha provides evidence in support of a role for corepressors in regulating cellular responses to agonists and antagonists. Mol. Endocrinol. 16, 1778–1792 500. Wijayaratne, A. L., Nagel, S. C., Paige, L. A., Christensen, D. J., Norris, J. D., Fowlkes, D. M.,

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501. Heldring, N., Nilsson, M., Buehrer, B., Treuter, E., and Carolina, N. (2004) Identification of Tamoxifen-Induced Coregulator Interaction Surfaces within the Ligand-Binding Domain of Estrogen Receptors. 24, 3445–3459

502. Aucoin, M. G., Mena, J. A., and Kamen, A. A. (2010) Bioprocessing of Baculovirus Vectors: A Review. Curr. Gene Ther. 10, 174–186

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