Cover Page The handle http://hdl.handle.net/1887/45594

Cover Page

The handle http://hdl.handle.net/1887/45594 holds various files of this Leiden University dissertation

Author: Holst, Stephanie

Title: Glycomic signatures of colorectal cancer

Issue Date: 2017-01-24

G ^lycomic S ^{iGnatureS of} c ^olorectal c ^ancer

Stephanie Holst

ISBN: 978-94-6295-562-2

©2016 Stephanie Holst. All rights reserved. No part of this book may be reproduced, stored in a retrieval system or transmitted in any form or by any means without permission of the author or the journals holding the copyrights of the published manuscripts. All published material was reprinted with permission.

The work presented in this thesis was performed at the Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, The Netherlands.

This work was supported by the European Union Seventh Framework Programme HighGlycan project, grant number: 278535.

Cover design: Alejo Bernal Arango Layout: Ana I. Belo

Printing: Proefschriftmaken.nl || Uitgeverij BOXPress

Proefschrift

ter verkrijging van

de graad van Doctor aan de Universiteit Leiden,

op gezag van Rector Magnificus prof.mr. C.J.J.M. Stolker, volgens besluit van het College voor Promoties

te verdedigen op dinsdag 24 januari 2017 Klokke10:00 uur

door

Stephanie Holst geboren te Kiel, Duitsland

in 1987

Glycomic Signatures of Colorectal Cancer

Promotores: Prof. Dr. M. Wuhrer Prof. Dr. A. M. Deelder

Co-promotor: Dr. Y. Rombouts (Institut de Pharmacologie et de Biologie Structurale, Université de Toulouse, CNRS, Toulouse, France)

Overige leden: Prof. Dr. J. Morreau Prof. Dr. R. A. E. M. Tollenaar Prof. Dr. P. Devilee

Prof. Dr. Y. van Kooyk (Department of Molecular Cell Biology and Immunology, VU University medical center, Amsterdam, The Netherlands)

Prof. Dr. G.-J. Boons (Chemical Biology and Drug Discovery, Departments of Pharmaceutical Sciences and Chemistry, Faculty of Sciences, Utrecht University, Utrecht, The Netherlands)

To all patients, relatives, and friends having to face cancer.

TABLE OF CONTENTS

Chapter 1: General Introduction

Chapter 2: Investigations on aberrant glycosylation of glycosphingolipids in colorectal cancer tissues using liquid chromatography and MALDI-TOF-MS

Chapter 3: N-glycosylation profiling of colorectal cancer cell lines reveals association of fucosylation with differentiation and CDX1/villin mRNA expression

Chapter 4: N-glycomic and transcriptomic changes associated with CDX1 expression in colorectal cancer cell lines

Chapter 5: N-glycomic profiling of pancreatic cancer cells with different metastatic behavior

Chapter 6: Linkage-specific in-situ sialic acid derivatization for N-glycan mass spectrometry imaging of FFPE tissues

Chapter 7: General Discussion

Appendix: List of Abbreviations Summary

Samenvatting

Curriculum Vitae

List of publications

Acknowledgements