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Light-Harvesting Complexes in Chlamydomonas reinhardtii:
Natali, A.
2017
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citation for published version (APA)
Natali, A. (2017). Light-Harvesting Complexes in Chlamydomonas reinhardtii: from in vitro to in vivo.
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Contents
1. Introduction ... 9
1.1. PHOTOSYNTHESIS ... 9
1.2. CHLAMYDOMONAS ... 11
1.3. PIGMENTS IN HIGHER PLANTS AND GREEN ALGAE ... 12
1.4. LIGHT HAVESTING COMPLEXES... 15
1.5. PHOTOPROTECTION ... 18
2. In vitro reconstitution of light-harvesting complexes of plants and green algae ... 25
2.1. INTRODUCTION ... 26
2.2. PROTOCOL ... 27
2.3. REPRESENTATIVE RESULTS ... 33
2.4. DISCUSSION ... 36
3. Characterization of the Major-Light Harvesting complexes (LHCBM) of the green alga Chlamydomonas reinhardtii ... 41
3.1. INTRODUCTION ... 42
3.2. MATERIALS AND METHOD ... 43
3.3. RESULTS ... 46
3.4. DISCUSSION ... 55
3.5. SUPPORTING INFORMATIONS ... 59
4. Light-harvesting Complexes (LHCs) cluster spontaneously in membrane environment leading to shortening of their excited state lifetime ... 61
4.1. INTRODUCTION ... 62
4.2. RESULTS ... 63
4.4. EXPERIMENTAL PROCEURES ... 73
5. Engineering a pH-regulated switch in the major light-harvesting complex of plants (LHCII): proof of principle ... 77
5.1. INTRODUCTION ... 78
5.2. MATERIALS AND METHODS ... 79
5.3. RESULTS AND DISCUSSIONS ... 80
5.4. CONCLUSIONS ... 84
5.5. SUPPORTING INFORMATIONS ... 85
6. Expression of recombinant LHCSR3 in C. reinhardtii and N. tabacum ... 91
6.1. INTRODUCTION ... 92
6.2. MATERIALS AND METHOD ... 93
6.3. RESULTS ... 95
6.4. DISCUSSION ... 100
6.5. CONTRIBUTIONS and ACKNOWLEDGEMENTS ... 102
Bibliography ... 105
Summary
