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Figure 1. Isolation and characterization of the primary PDAC cell line NFCR01. (

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Figure 1. Isolation and characterization of the primary PDAC cell line NFCR01. (A) Micrograph of a pure population of epithelial PDAC cells, as obtained via contrast phase light microscopy (Original magnification 20×, scale bar = 50μm). (B) Micrographs of laser microdissected epithelial cells from a PDAC fresh tissue to perform DNA extraction (Original magnification 10×). (C) K-ras gene mutation analysis of isolated cells showing G216T mutation in codon 12 of exon 2.

Published in: Claudio Ricci; Carlos Mota; Stefania Moscato; Delfo D’Alessandro; Stefano Ugel; Silvia Sartoris; Vincenzo Bronte; Ugo Boggi; Daniela Campani; Niccola Funel; Lorenzo Moroni; Serena Danti;

Biomatter 2014, 4,

DOI: 10.4161/21592527.2014.955386 Copyright © 2014 The Author(s)

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