Fluorescence Polarization Activity- Based Protein Profiling on

There are two types of probes for the detection of active proteins (Figure 1b): (1) one-step probes make use of a compound with a detection tag already installed and (2) two-step

Through parallel application of both the competitive ABPP method and enzymatic assay in lysates of cultured cells, zebrafish (Danio rerio) larvae, and brains of mice treated in

Title: Activity-based protein profiling in drug-discovery Issue Date: 2019-05-28... Activity-based protein profiling in

26 − 29 To determine the selectivity of compound 1 on endogenously expressed serine hydrolases, we performed a competitive ABPP experiment in mouse brain proteomes using

21,28,29 Here, a multiplexed ABPP assay with ABP (3) and MB064 was developed and used to study endocannabinoid hydrolase activity and to pro file inhibitors on activity and

Norbornene probe 1 was successfully synthesized as a two-step ABP for labeling of DAGL α using an IEDDA ligation in living cells and compared to a click reaction and direct probe

The a-Glucosidase Probes Label a Distinct Subproteome To test the labeling by the new probes, Arabidopsis leaf extracts were labeled with JJB382 and JJB70, which differ in

L -ido- con figured compounds 10 and 12 are over 100 000-fold selective for GBA2 over GBA1, which is a signi ficant improvement compared to previously reported inhibitors showing