Fluorescence Polarization Activity- Based Protein Profiling on

L -ido- con figured compounds 10 and 12 are over 100 000-fold selective for GBA2 over GBA1, which is a signi ficant improvement compared to previously reported inhibitors showing

Title: Activity-based protein profiling in drug-discovery Issue Date: 2019-05-28... Activity-based protein profiling in

Through parallel application of both the competitive ABPP method and enzymatic assay in lysates of cultured cells, zebrafish (Danio rerio) larvae, and brains of mice treated in

26 − 29 To determine the selectivity of compound 1 on endogenously expressed serine hydrolases, we performed a competitive ABPP experiment in mouse brain proteomes using

21,28,29 Here, a multiplexed ABPP assay with ABP (3) and MB064 was developed and used to study endocannabinoid hydrolase activity and to pro file inhibitors on activity and

Norbornene probe 1 was successfully synthesized as a two-step ABP for labeling of DAGL α using an IEDDA ligation in living cells and compared to a click reaction and direct probe

There are two types of probes for the detection of active proteins (Figure 1b): (1) one-step probes make use of a compound with a detection tag already installed and (2) two-step

The a-Glucosidase Probes Label a Distinct Subproteome To test the labeling by the new probes, Arabidopsis leaf extracts were labeled with JJB382 and JJB70, which differ in