Single cell biochemistry to visualize antigen presentation
and drug resistance
Griekspoor, A.C.
Citation
Griekspoor, A. C. (2006, November 1). Single cell biochemistry to visualize antigen presentation and drug resistance. Retrieved from
https://hdl.handle.net/1887/4962
Version: Corrected Publisher’s Version
License: Licence agreement concerning inclusion of doctoralthesis in the Institutional Repository of the University of Leiden
Downloaded from: https://hdl.handle.net/1887/4962
Single Cell Biochemistry to
visualize Antigen Presentation
and Drug Resistance
Single Cell Biochemistry to visualize
Antigen Presentation and Drug Resistance
Proefschrift ter verkrijging van
de graad van Doctor aan de Universiteit Leiden, op gezag van de Rector Magnificus Dr. D.D. Breimer,
hoogleraar in de faculteit der Wiskunde en Natuurwetenschappen en die der Geneeskunde,
volgens besluit van het College voor Promoties te verdedigen op woensdag 1 november 2006
klokke 15.00 uur door
Alexander Christiaan Griekspoor geboren te Amstelveen
Promotiecommissie
Promotor: Prof. Dr. J.J. Neefjes
Copromoter: Dr. R.J.A.M. Michalides
Nederlands Kanker Instituut
Referent: Prof. Dr. W.H. Moolenaar
Overige Leden: Dr. S.M. van Ham
Sanquin Research
Dr. K. Jalink
Nederlands Kanker Instituut
Prof. Dr. T.H.M. Ottenhoff Prof. Dr. P.J. Peters
Vrije Universiteit, Amsterdam
Prof. Dr. B. van de Water Prof. Dr. E.J.H.J. Wiertz
Reproduction: Ponsen & Looijen BV, Wageningen
‘‘
Wat je zoekt is niet te vinden,
‘‘
wat je vindt is wat je zocht
—anoniem
Table of Contents
PrefacePreface - Single Cell Biochemistry 11
Part 1 - Antigen Presentation
Review
Chapter 1 Presenting Antigen Presentation in living cells using 15 biophysical techniques
Current Opinion in Microbiology
Review
Chapter 2 MHC class I alleles and their exploration of the antigen 25 processing machinery
Immunological Reviews
Article
Chapter 3 Peptide diffusion, protection, and degradation in nuclear 47 and cytoplasmic compartments before antigen
presentation by MHC class I
Immunity
Review
Chapter 4 Chaperoning antigen presentation by MHC class II 65 molecules and their role in oncogenesis
Advances in Cancer Research
Article
Chapter 5 Spatial separation of HLA-DM/HLA-DR interactions within 85 MIIC and phagosome induced immune escape
Immunity
Articles
Chapter 6 Immune escape and spreading of Salmonella after specific 111 B Cell Receptor-mediated uptake
B cell activation and induction of acquired immunity through 125 BCR-mediated phagocytosis of Salmonella
Submitted
Summary
Chapter 7 Summary and Discussion - Antigen Presentation 137
Table of Contents
Continued from previous page...
Part 2 - Drug Resistance
Review
Chapter 8 Visualizing the action of steroid hormone receptors 143 in living cells
Submitted
Article
Chapter 9 Tamoxifen resistance by a conformational arrest of 159 the Estrogen Receptor α after PKA activation in breast cancer
Cancer Cell
Article
Chapter 10 A FRET profile of modifications in Estrogen Receptor α 177 associated with resistance to anti-estrogens
Submitted
Article
Chapter 11 Protein Kinase A-induced tamoxifen resistance through 195 altered orientation of Estrogen Receptor α towards
co-activator SRC-1
Submitted
Summary
Chapter 12 Summary and Discussion - Drug Resistance 211
Epilogue
Epilogue - Single Cell Biochemistry 219
