DNA binding by the Rev1 BRCT region : implications for biological and structural function
Groote, F.H. de
Citation
Groote, F. H. de. (2011, February 9). DNA binding by the Rev1 BRCT region : implications for biological and structural function. Retrieved from https://hdl.handle.net/1887/16451
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DNA binding by the Rev1 BRCT region:
Implications for Structural and Biological Function
Proefschrift
Ter verkrijging van
de graad van Doctor aan de Universiteit Leiden
op gezag van Rector Magnificus Prof. Mr. P.F. van der Heijden, volgens besluit van het College voor Promoties
te verdedigen op woensdag 9 februari 2011.
Klokke 15:00 uur
door
Frederik Hendrik de Groote
Geboren te Emmen in 1980
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Promotiecommissie
Promotor: Prof.dr. J. Brouwer.
Co-Promotor: Dr. G. Siegal.
Overige leden: Prof.dr. J.E. Sale (Cambridge University, UK) Prof.dr. M. Ubbink.
Dr. J.G. Jansen.
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“You live and learn. At any rate, you live!’”
Douglas Adams.
English humorist and science fiction novelist (1952-2001)
Voor mijn (groot)ouders
4 Cover design: F.H. de Groote
Printed by Wöhrmann Print Service, Zutphen.
ISBN 978-90-8570-719-6 F.H. de Groote 2011
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Contents
List of Abbreviations 6
Chapter I General Introduction 9
Chapter II DNA Binding Properties of the Rev1 BRCT Region 53 Chapter III Determination of Amino Acid Requirements for DNA
Binding by the Rev1 BRCT Region 79
Chapter IV Implications of BRCT Mediated DNA Binding:
Cooperative Binding of Two Primer Termini 99 Chapter V Determination of the Cellular Role of the Rfc1 BRCT
Region
117
Chapter VI General Discussion, Conclusion and Future Prospects 139
Summary 151
Samenvatting 154
Dankwoord 158
CV 161
List of publications 162
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List of Abbreviations
9-1-1 Rad9- Rad1- Hus1 Complex 20NT Amino acids 1-20 of Rev1
aa amino acids
BER Base Excision Repair
BRCA1 Breast Cancer Type 1 Susceptibility Protein BPDE Benzo[a]pyrene diolepoxide
BRCT BRCA1 C-Terminal Domain CIP Calf Intestinal Phosphatase CPD Cyclobutane Pyrimidine Dimer DTD Deoxycytidyl Transferase Domain EMSA Electrophoretic Mobility Shift Assay FAN1 Fanconi Associated Nuclease 1 Fen1 Flap Endonuclease 1
HR Homologous Recombination ICL Interstrand Cross link
MCM Mini-Chromosome Maintenance MMR Mismatch Repair
NER Nucleotide Excision Repair NIR Nucleotide Incision Repair NTR N-Terminal Region
ORC Origin Recognition Complex
P Phosphorylated
PAD Polymerase Associated Domain PCNA Proliferating Cell Nuclear Antigen PID Polymerase Interaction Domain
Pol Polymerase
7 RID Rev1 Interaction Domain RFC Replication Factor C RPA Replication Protein A SDM Site directed mutagenesis SHP Single hairpin
TLS Translesion Synthesis UBM Ubiquitin Binding Motif UBZ Ubiquitin Binding Zinc Finger
WRN Werner Syndrome
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