The BRCT domain from the large subunit of human Replication Factor
C
Kobayashi, Masakazu
Citation
Kobayashi, M. (2006, September 6). The BRCT domain from the large subunit of human
Replication Factor C. Retrieved from https://hdl.handle.net/1887/4546
Version: Corrected Publisher’s Version
The BRCT domain from the large subunit of
human Replication Factor C: Protein-DNA
complex determined by NMR and mutagenesis.
PROEFSCHRIFT
TER VERKRIJGING VAN DE GRAAD VAN DOCTOR AAN DE UNIVERSITEIT LEIDEN, OP GEZAG VAN DE RECTOR MAGNIFICUS DR. D. D. BREIMER, HOOGLERAAR IN DE FACULTEIT DER WISKUNDE EN NATUURWETENSCHAPPEN EN DIE DER GENEESKUNDE, VOLGENS BESLUIT VAN HET COLLEGE VOOR PROMOTIES TE VERDEDIGEN OP WOENSDAG 6 SEPTEMBER 2006 TE KLOKKE 16:15 UUR
Door
Masakazu Kobayashi
Promotie commissie
Promotor : Prof. Dr. G.W. Canters Co-Promoter: Dr. G. Siegal
Referent : Prof. Dr. P. M. Burgers Overige Leden :
Prof. Dr. J. Brouwer Prof. Dr. R. Boelens
Front and rear covers: The layout was designed by Emanuela Lonardi using the original
Contents
Chapter 1 General Introduction 7
Chapter 2 Characterization of the DNA binding and Structural Properties of the BRCT region of the p140 subunit of human replication Factor C
39
Chapter 3 Amino acid determinants for DNA binding by the BRCT region of human RFC p140
65
Chapter 4 1H,15N and 13C resonance assignments and secondary structure determination of the BRCT Region of the large subunit of human Replication Factor C, bound to DNA
81
Chapter 5 Structure of the BRCT domain from RFC p140: A model Protein-DNA complex determined by NMR and mutagenesis data
93
Chapter 6 General discussions and future prospective 133
Summary in English 143
Samenvatting 146
Appendices: Colour figures 148
List of publications 155
Curriculum Vitae 156
