University of Groningen
Bacillus subtilis: sporulation, competence and the ability to take up fluorescently labelled DNA
Boonstra, Mirjam
IMPORTANT NOTE: You are advised to consult the publisher's version (publisher's PDF) if you wish to cite from it. Please check the document version below.
Document Version
Publisher's PDF, also known as Version of record
Publication date: 2017
Link to publication in University of Groningen/UMCG research database
Citation for published version (APA):
Boonstra, M. (2017). Bacillus subtilis: sporulation, competence and the ability to take up fluorescently labelled DNA. Rijksuniversiteit Groningen.
Copyright
Other than for strictly personal use, it is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), unless the work is under an open content license (like Creative Commons).
Take-down policy
If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim.
Downloaded from the University of Groningen/UMCG research database (Pure): http://www.rug.nl/research/portal. For technical reasons the number of authors shown on this cover page is limited to 10 maximum.
Bacillus subtilis: sporulation,
competence and the ability to
take up fluorescently labelled
DNA
Mirjam Boonstra
2017
The research presented in this thesis was carried out in the department of Moleclar Genetics, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), Faculty of Science and Engineering, University of Groningen, the Netherlands. The research done by M. Boonstra was funded by the Simon Stevin Meester price awarded to O.P. Kuipers by Stichting Technische Wetenschappen (STW), 13344.
Printing of this thesis was financially supported by the Graduate School of Sciences, University of Groningen.
Cover design by M. Boonstra Printed by Ipskamp Printing
© Mirjam Boonstra, 2017, Lelystad, the Netherlands
ISBN (printed version): 978-94-034-0231-4 ISBN (electronic version): 978-94-034-0230-7
Bacillus subtilis: sporulation, competence and the ability to take up
fluorescently labelled DNA
Proefschrift
ter verkrijging van de graad van doctor aan de Rijksuniversiteit Groningen
op gezag van de
rector magnificus prof. dr. E. Sterken en volgens besluit van het College voor Promoties.
De openbare verdediging zal plaatsvinden op vrijdag 8 december 2017 om 16.15 uur
door
Mirjam Boonstra
geboren op 28 juli 1983 te Delfzijl
Promotores
Prof. dr. O.P. Kuipers Prof. dr. J.W. Veening
Beoordelingscommissie Prof. dr. J.M. van Dijl Prof. dr. L.W. Hamoen Prof. dr. I. Mandič-Mulec
Content
Chapter 1: General Introduction 9-43
Chapter 2: Spo0A regulates chromosome copy number during sporulation by directly binding to the origin of replication in B. subtilis
47-75
Chapter 3: Analyses of the competent and non-competent subpopulations of B. subtilis reveal yhfW, yhxC and ncRNAs as novel players in competence
79-117
Chapter 4: Following the fate of incoming DNA during natural transformation of Bacillus subtilis with fluorescently labelled DNA
121-145
Chapter 5: Visualizing and tracking down the uptake and integration of labelled DNA during competence of Bacillus subtilis by time-lapse microscopy
149-168
Chapter 6: Summary & Discussion 171-183
Chapter 7: Nederlanse Samenvatting voor niet wetenschappers
