University of Groningen
Comprehensive characterization of Escherichia coli isolated from urine samples of
hospitalized patients in Rio de Janeiro, Brazil
da Cruz Campos, Ana
DOI:
10.33612/diss.111520622
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Publication date:
2020
Link to publication in University of Groningen/UMCG research database
Citation for published version (APA):
da Cruz Campos, A. (2020). Comprehensive characterization of Escherichia coli isolated from urine
samples of hospitalized patients in Rio de Janeiro, Brazil: the use of next generation sequencing
technologies for resistance and virulence profiling and phylogenetic typing. University of Groningen.
https://doi.org/10.33612/diss.111520622
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Stellingen
Behorende bij het proefschrift
Comprehensive characterization of
Escherichia coli
isolated from urine
samples of hospitalized patients in Rio de Janeiro, Brazil
The use of next generation sequencing technologies
for resistance and virulence profiling and phylogenetic typing
1. E. coli high-risk clones are linked to an increase in antibiotic resistance rates and are responsible for spread of specific resistant determinants, such as the blaCTX-M-15 gene, in Rio de Janeiro, Brazil. (This Thesis)
2. In contrast to previous reports on other countries, successful E. coli lineages such as ST69 and ST73 that are responsible for urinary tract infections in hospitalized patients in Rio de Janeiro, were not associated with a multidrug resistance profile. (This Thesis)
3. The multidrug resistance profile in E. coli high-risk clones is strongly associated with the IncF plasmids and their resistance cassettes. (This Thesis)
4. Among ST131 E. coli sublineages, specific mobile genetic elements allow a balanced interplay between their resistance and virulence properties that seems essential for their success. (This Thesis) 5. Fosfomycin heteroresistance is an under-investigated and under-diagnosed problem, particularly
among multidrug resistant E. coli, and can be caused by several independent molecular mechanisms.
(This Thesis)
6. Fosfomycin heteroresistance can affect the eradication of biofilms, and thereby affects the efficiency of this antibiotic against intracellular bacterial communities (biofilm-like structures). (This thesis) 7. ST131 E. coli isolates are not intrinsically resistant to bacteriocins and are more sensitive to
bacteriocins produced by isolates belonging to other lineages. (This Thesis)
8. E. coli belonging to the H22-ST131 sublineage, often considered as foodborne uropathogens, are able to produce bacteriocins that may act as antimicrobial or killing peptides, directly inhibiting competing strains or pathogens. (This Thesis)
9. Elderly patients and patients suffering from comorbidities have a higher risk of getting a urinary tract infection caused by multidrug resistant or extended spectrum beta-lactamase -producing high-risk E. coli clones. (This Thesis)
10. “The more we increase our knowledge, the more evident our ignorance becomes.” (John F. Kennedy)
11. “The task is not so much to see what no one has seen, but to think what no one has yet thought about what everyone sees.” (Arthur Schopenhauer)
12. “I have no special talent. I am only passionately curious” (Albert Einstein)
