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University of Groningen Fluorescent Nanodiamonds as Free Radical Sensors in Aging Yeast Cells van der Laan, Kiran

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University of Groningen

Fluorescent Nanodiamonds as Free Radical Sensors in Aging Yeast Cells van der Laan, Kiran

DOI:

10.33612/diss.112906297

IMPORTANT NOTE: You are advised to consult the publisher's version (publisher's PDF) if you wish to cite from it. Please check the document version below.

Document Version

Publisher's PDF, also known as Version of record

Publication date: 2020

Link to publication in University of Groningen/UMCG research database

Citation for published version (APA):

van der Laan, K. (2020). Fluorescent Nanodiamonds as Free Radical Sensors in Aging Yeast Cells: a baker’s yeast response to small diamonds with great potential!. Rijksuniversiteit Groningen.

https://doi.org/10.33612/diss.112906297

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Downloaded from the University of Groningen/UMCG research database (Pure): http://www.rug.nl/research/portal. For technical reasons the number of authors shown on this cover page is limited to 10 maximum.

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Fluorescent Nanodiamonds as Free Radical Sensors

in Aging Yeast Cells

A baker’s yeast response to small diamonds with great potential!

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Fluorescent Nanodiamonds as Free Radical Sensors in Aging Yeast cells

By Kiran J. van der Laan

This work is part of the research programme of the Foundation for Fundamental Research on Matter (FOM), which is part of the Dutch Research Council (NWO).

Copyright ©2019 by Kiran J. van der Laan Printed by Ipskamp Printing B.V.

ISBN (printed version): 978-94-034-2358-6 ISBN (electronic version): 978-94-034-2357-9

Fluorescent Nanodiamonds as Free

Radical Sensors in Aging Yeast Cells

A baker’s yeast response to small diamonds with great potential!

Proefschrift

ter verkrijging van de graad van doctor aan de Rijksuniversiteit Groningen

op gezag van de

rector magnificus prof. dr. C. Wijmenga en volgens besluit van het College voor Promoties.

De openbare verdediging zal plaatsvinden op maandag 10 februari 2020 om 11.00 uur

door

Kiran Jans van der Laan

geboren op 7 september 1991

te Eindhoven

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539807-L-bw-Laan 539807-L-bw-Laan 539807-L-bw-Laan 539807-L-bw-Laan Processed on: 21-1-2020 Processed on: 21-1-2020 Processed on: 21-1-2020

Processed on: 21-1-2020 PDF page: 3PDF page: 3PDF page: 3PDF page: 3

Fluorescent Nanodiamonds as Free Radical Sensors in Aging Yeast cells

By Kiran J. van der Laan

This work is part of the research programme of the Foundation for Fundamental Research on Matter (FOM), which is part of the Dutch Research Council (NWO).

Copyright ©2019 by Kiran J. van der Laan Printed by Ipskamp Printing B.V.

ISBN (printed version): 978-94-034-2358-6 ISBN (electronic version): 978-94-034-2357-9

Fluorescent Nanodiamonds as Free

Radical Sensors in Aging Yeast Cells

A baker’s yeast response to small diamonds with great potential!

Proefschrift

ter verkrijging van de graad van doctor aan de Rijksuniversiteit Groningen

op gezag van de

rector magnificus prof. dr. C. Wijmenga en volgens besluit van het College voor Promoties.

De openbare verdediging zal plaatsvinden op maandag 10 februari 2020 om 11.00 uur

door

Kiran Jans van der Laan

geboren op 7 september 1991

te Eindhoven

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539807-L-bw-Laan 539807-L-bw-Laan 539807-L-bw-Laan 539807-L-bw-Laan Processed on: 21-1-2020 Processed on: 21-1-2020 Processed on: 21-1-2020

Processed on: 21-1-2020 PDF page: 4PDF page: 4PDF page: 4PDF page: 4

Promotor

Prof. dr. R. Schirhagl

Copromotor

Dr. A.L. Mzyk

Beoordelingscommissie

Prof. dr. U.L.M. Eisel

Prof. dr. M. Nesladek Prof. dr. G. de Haan

Table of content

Chapter 1 – General Introduction

Chapter 2 – Generally Applicable Transformation Protocols for

Fluorescent Nanodiamond Internalization into Cells

Chapter 3 – Toward using Fluorescent Nanodiamonds to Study

Chronological Aging in Saccharomyces cerevisae

Chapter 4 – Oxidative Stress Response of Aging Yeast Cells in Response

to Internalization of Fluorescent Nanodiamond Biosensors

Chapter 5 – Nanodiamonds and Their Applications in Cells Chapter 6 – Nanodiamonds for In Vivo Applications

Chapter 7 – General discussion Chapter 8 – Supplementary documents

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539807-L-bw-Laan 539807-L-bw-Laan 539807-L-bw-Laan 539807-L-bw-Laan Processed on: 21-1-2020 Processed on: 21-1-2020 Processed on: 21-1-2020

Processed on: 21-1-2020 PDF page: 5PDF page: 5PDF page: 5PDF page: 5

Promotor

Prof. dr. R. Schirhagl

Copromotor

Dr. A.L. Mzyk

Beoordelingscommissie

Prof. dr. U.L.M. Eisel

Prof. dr. M. Nesladek Prof. dr. G. de Haan

Table of content

Chapter 1 – General Introduction

Chapter 2 – Generally Applicable Transformation Protocols for

Fluorescent Nanodiamond Internalization into Cells

Chapter 3 – Toward using Fluorescent Nanodiamonds to Study

Chronological Aging in Saccharomyces cerevisae

Chapter 4 – Oxidative Stress Response of Aging Yeast Cells in Response

to Internalization of Fluorescent Nanodiamond Biosensors

Chapter 5 – Nanodiamonds and Their Applications in Cells Chapter 6 – Nanodiamonds for In Vivo Applications

Chapter 7 – General discussion Chapter 8 – Supplementary documents

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Processed on: 21-1-2020 PDF page: 6PDF page: 6PDF page: 6PDF page: 6

Chapter 1

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