From Fetus towards Adult
Maturation and functional analysis of pluripotent stem cell-derived cardiomyocytes
Marcelo Catarino Ribeiro
Cover Page
The handle http://hdl.handle.net/1887/43479 holds various files of this Leiden University dissertation
Author: Catarino Ribeiro, Marcelo
Title: From fetus towards adult : maturation and functional analysis of pluripotent stem cell-derived cardiomyocytes
Issue Date: 2016-10-13
Promotor: Prof. Dr. Christine Mummery Co-Promotor: Prof. Dr. Robert Passier Promotiecommissie:
- Prof. dr. R.C. Hoeben
- Prof.dr. T. Schmidt (Leiden Institute of Physics) - Prof. dr. M.J.T.H. Goumans
- Dr. E. Danen (LACDR)
Colophon
From Fetus towards Adult - Maturation and functional analysis of pluripotent stem cell-de- rived cardiomyocytes
This thesis was prepared at the Department of Anatomy & Embryology of the Leiden Uni- versity Medical Center, Leiden, The Netherlands
Copyright © 2016 Marcelo C. Ribeiro
All rights reserved. No part of this book may be reproduced or transmitted, in any form or by any means, without permission in writing of the author. The copyright of the article that have been published or have been accepted for publication has been transferred to the respective journals.
The research described in this thesis as well as its publication was supported by a grant of Fundamenteel Onderzoek der Materie (FOM).
Financial support by the Dutch Heart Foundation and by the Stichting Proefdiervrij for the publication of this thesis is gratefully acknowledged.
ISBN: 978-90-9029874-0
Cover: Roeland Reitsema, Matthew Schriock and Marcelo C. Ribeiro Design and Lay-out: Marcelo C. Ribeiro
Printing: Uitgeverij BOXpress || proefschriftmaken.nl
From Fetus towards Adult
Maturation and functional analysis of pluripotent stem cell-derived cardiomyocytes
Proefschrift
ter verkrijging van
de graad van Doctor aan de Universiteit Leiden, op gezag van Rector Magnificus prof.mr. C.J.J.M. Stolker,
volgens besluit van het College voor Promoties te verdedigen op donderdag 13 oktober 2016 klokke 16.15 uur
door
Marcelo Catarino Ribeiro geboren op 18 oktober
1987, Lissabon
Esta tese é dedicada aos meus pais
Chapter 1 Chapter 2 Chapter 3
Chapter 4
Chapter 5 Chapter 6
Chapter 7
Chapter 8
Contents
General Introduction Scope of this thesis
Mimicking the in vivo microenvironment in a cell culture dish
Functional maturation of human pluripotent stem cell derived cardiomyocytes in vitro – correlation between contraction force and electrophysiology
Biomaterials. 2015; 51:138–50
Contractile defect caused by mutation in MYBPC3 revealed under conditions optimized for human PSC-cardiomyocyte function
Adapted from CellReports. 2015; (13):1–13
Alpha-Actinin-mCherry fusion protein – Building a paramount new tool for cardiomyocyte analysis
Hypertrophic Cardiomyopathy - Impaired sarcomeric motion and contractile dysfunction caused by defective MYBPC3 in hPSC derived cardiomyocytes
Expansion and developmental patterning of human PSC-derived cardiovascular progenitors
Nat Biotechnol. 2015; 33(9):1–12
General discussion and future perspectives
Summary/ Resumo/ Samenvatting Acknowledgements
Curriculum Vitae List of Publications
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