Infectious disease studies in zebrafish : the fish pathogen Edwardsiella tarda as a model system

Chapter 4 Expression profiling of the response to Edwardsiella tarda infection in a zebrafish myd88 knockout

Cover: Scanning electron microscopy image by Gerda Lamers of Edwardsiella tarda bacteria on the skin of a zebrafish embryo.. Printed by: Ridderprint, Ridderkerk, The Netherlands

In this thesis, we set up a model to study host-pathogen interactions using the zebrafish embryo as a model host and the bacterial species Edwardsiella tarda, a known

As an example of the usefulness of these datasets we compared single nucleotide polymorphisms in several gene clusters that are associated with virulence, also using

Induction of il1b and mmp9 was consistently observed in embryos that had been systemically infected by intravenous injection, while the early transcriptional induction

In conclusion, microarray analysis showed that the infection-induction of a large group of immune response genes was reduced in myd88 -/- embryos compared to

Since the immersion assay was shown not to be suitable for analysis on the level of single embryos and did not produce a clear immune response, we used intravenous

Terwijl cyp1a zoals verwacht niet was geïnduceerd en irg1l geïnduceerd was in alle embryo’s, mogelijk als een reactie op losgelaten bacteriële stoffen, waren mmp9 en il1b