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Cover Page

The handle

http://hdl.handle.net/1887/92370

holds various files of this Leiden University

dissertation.

Author: Poulcharidis, D.

(2)

Emerging Approaches

to Study Cell-Cell Interactions

PROEFSCHRIFT

ter verkrijging van

de graad van Doctor aan de Universiteit Leiden,

op gezag van Rector Magnificus prof. mr. C. J. J. M.

Stolker,

volgens het besluit van het College voor Promoties

te verdedigen op woensdag 3 juni 2020

klokke 15:00 uur

door

∆ημήτριος Πουλχαρίδης

Dimitrios Poulcharidis

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(4)

Promotiecommissie

Promotor: Prof.dr. A. Kros

Co-promotor: Dr S.I. van Kasteren

Overige leden: Prof.dr. H.S. Overkleeft (Voorzitter) Prof.dr. J. Brouwer (Secretaris)

Prof.dr. J.A. Bouwstra (Universiteit Leiden) Dr. D. Claessen (Universiteit Leiden)

Dr. R. Eelkema (Technische Universiteit Delft) Prof.dr. J.J.L.M. Cornelissen (Universiteit Twente)

Cover design: HaisonokViktoriia/Shutterstock.com Printed by: Ipskamp Printing

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Table of Contents

Chapter 1 5

Aim and outline of this thesis

Chapter 2 9

Chemical approaches to enhance cell-cell contact

Chapter 3 43

A flow cytometry assay to quantify intercellular exchange of membrane components

Chapter 4 75

Identifying membrane lipid exchange between immune cells

Chapter 5 93

In vitro supramolecular modification of L-form bacteria

using lipidated coiled-coil peptides

Chapter 6 123

In vivo and in vitro evaluation of a phospholipid ether

analogue for the detection of tumours

Summary 145

Summary in Greek 149

List of publications 155

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