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The handle http://hdl.handle.net/1887/23622 holds various files of this Leiden University dissertation
Author: Petrova, I.M.
Title: Non-canonical Wnt signaling via the Ryk and Ror receptors in the drosophila nervous system
Issue Date: 2014-02-12
Non-canonical Wnt Signaling via the Ryk and Ror Receptors in the
Drosophila Nervous System
Iveta Miroslavova Petrova
Ивета Мирославова Петрова
Printed by: Proefschriftmaken.nl || Uitgeverij BOXPress Published by: Uitgeverij BOXPress, ‘s-Hertogenbosch Cover design: Vesela M. Petrova
ISBN: 978-90-8891-811-7
Financial support for the printing of the thesis: MCB department of the LUMC and ABN AMRO Bank N.V.
The studies described in this thesis were performed at the Department of Molecular Cell Biology, Leiden University Medical Center, Leiden, The Netherlands and were supported by ZonMw Topgrant #91210058 from N.W.O.
Non-canonical Wnt Signaling via the Ryk and Ror Receptors in the Drosophila Nervous
System
Proefschrift
ter verkrijging van
de graad van Doctor aan de Universiteit Leiden, op gezag van Rector Magnificus Prof. Mr. C.J.J.M. Stolker,
volgens besluit van het College voor Promoties te verdedigen op woensdag 12 februari 2014
klokke 16:15 uur
door
Iveta Miroslavova Petrova
geboren te Sofia (Bulgarije) in 1984
Promotiecommissie
Promotor: Prof. Dr. J. N. Noordermeer
Co-promotor: Dr. L. G. Fradkin Overige leden: Prof. Dr. H.J. Tanke
Prof. Dr. M. J. Malessy Dr. J. M. Dura1
1Institute of Human Genetics, CNRS, Montpellier, France.
Contents
Chapter 1: Introduction 7 1.1. Wnt Signaling through the Ror Receptor in the Nervous System 8 1.2. General Introduction 22
1.2.1. Drosophila as a Model Organism for Neurobiological Studies 23
1.2.2. Development of the Embryonic Nervous System of Drosophila 25
1.2.3. The Drosophila Neuromuscular Junction 28 1.2.4. Electrophysiology at the Larval NMJ of Drosophila 31 1.2.5. The Olfactory System of the Drosophila Adult 34 1.2.6. Drosophila Mushroom Bodies in the Adult Fly 35
1.2.7. Wnt pathways 37
1.3. Outline of the Thesis 43
Chapter 2: Drosophila Ror Controls the Patterning of Embryonic Glia and Longitudinal Axon Pathways and Synaptic Transmission at
the Larval Neuromuscular Junction 55 Chapter 3: Homodimerization of the Wnt Receptor DERAILED
Recruits the Src Family Kinase SRC64B 85
Chapter 4: Wnt5 and Drl Gradients Pattern the Drosophila Olfactory Dendritic Map 101 Chapter 5: Extrinsic Drl Guides Drl-2-expressing Drosophila Mushroom Body
Axons by Localizing Wnt5 139
Chapter 6: Discussion 167
Summary 177
Samenvatting 180
Abbreviations 183
Curriculum Vitae 185
List of Publications 186
Acknowledgements 187
